超声波辅助提取月柿原花青素及其纯化

Ultrasonic Extraction and Purification of Proanthocyanidins from Persimmon

研究了超声波辅助提取月柿生理落果原花青素工艺。适宜工艺条件为:超声波功率150 W、提取溶液乙醇体积分数70%、料液比(g∶mL)1∶12、提取时间15 min、提取温度45℃。通过对9种大孔吸附树脂的研究,确定LS300树脂为优选纯化树脂。研究了原花青素在LS300树脂上的吸附行为,静态吸附研究表明,其吸附等温曲线符合Freundlich等温吸附方程,吸附平衡的时间约为5h。乙醇浓度梯度洗脱试验表明,原花青素主要易被体积分数20%、40%乙醇溶液洗脱,两部分原花青素质量分数分别为86.5%、92.3%。

The optimum ultrasonic extraction process of proanthocyanidins was studied. Optimal extracting condition was confirmed as follows ： the rate of power of ultrasonic was 100% , alcohol content was 70% , the ratio of liquid to material was 1 ： 12, the extraction time was 15mins and the extraction temperature was 45℃. Choose LS300 resin as the material for the purification. The absorbability of LS300 resin was studied. The results of static adsorption showed that the adsorption isotherm could be correlated with Freundlich. The adsorption equilibrium time was about 5h. The experiment of concentration gradient elution by ethanol solution showed that the most proanthoeyanidins were concentrated on two parts： the 20% and 40%. The mass fractions of two parts were 86.5% and 92.3%.