黄芩甙对牙周炎小鼠血清白细胞介素-6和白细胞介素-4水平的影响

Effects of baicalin on serum levels of IL-6 and IL-4 in mouse periodontitis

目的:观察黄芩甙对小鼠实验性牙周炎血清白细胞介素-6(IL-6)、白细胞介素-4(IL-4)的影响,探讨黄芩甙对牙周炎的免疫调节作用机制。方法:建立小鼠实验性牙周炎模型,并予以黄芩水提取物灌胃治疗。实验采用SPF级12周龄雄性KM小鼠27只,随机分为3组:正常对照组;实验性牙周炎组:参照Kimura等的方法,复制小鼠实验性牙周炎模型;黄芩甙治疗组:复制小鼠实验性牙周炎模型,并于建模后5周开始用黄芩水提取物灌胃。各组小鼠分别在建模后第4、6、8周分批处死,各时点每组处死动物数为3只。制作牙体牙周组织联合切片,观察牙周的组织学改变;采用ELISA法检测实验性牙周炎小鼠外周血IL-6和IL-4的水平。结果:实验性牙周炎组牙周组织表现为中等程度的炎症破坏,而黄芩甙治疗组牙周组织炎症程度较牙周炎组明显减轻,修复反应明显;牙周炎组血清IL-6水平显著高于正常对照组和黄芩甙治疗组(P<0.01);黄芩甙治疗组血清IL-6的含量在6和8周时显著低于牙周炎组(P<0.01);牙周炎组血清IL-4水平显著低于正常对照组和黄芩甙治疗组(P<0.01),而黄芩甙治疗组血清IL-4的含量在6和8周时显著高于牙周炎组(P<0.01)。结论:牙周炎的发病过程与Th1/Th2细胞因子的失调密切相关,表现为IL-6升高,IL-4降低;黄芩甙通过降低牙周炎IL-6的含量,增加IL-4的分泌量,纠正牙周炎Th1/Th2细胞因子的失调状况,对小鼠实验性牙周炎有明显的治疗效果。

AIM： To investigate the effect of baicalin on experimental periodontitis in mouse model by comparing the histological changes in periodontal tissues and serum levels of inter leukin（IL）-6/IL-4 in mice,and to analyze the role of baicalin in immune regulation and anti-inflammatory mechanisms.METHODS： Twenty-seven male Kunming mice（SPF grade,12-week-old） were randomly divided into 3 groups.The naive mice were used in normal control group.In experimental periodontitis group,the periodontitis model was produced by ligature of braided silk around the first maxillary molar and inoculation with putative periodontopathic bacteria.Five weeks after the ligature,the mice were fed with 10% glucose,and gavaged with distilled water.In baicalin treatment＋periodontitis group,the periodontitis model was induced as above,then gavaged with baicalin at the beginning of the fifth week after the ligature.The mice were sacrificed at week 4,6 and 8.The histological changes of the periodontal tissues were observed under microscope with hematoxylin and eosin（HE） staining.The serum level of IL-6 and IL-4 in the mice were determined by ELISA.RESULTS： The periodontal tissues showed moderate inflammatory damages in experimental periodontitis group.The periodontal destruction was significantly reduced in baicalin treatment＋periodontitis group.The serum level of IL-6 in experimental periodontitis group was significantly higher than that in control group and baicalin treatment＋periodontitis group（P〈0.01）,and the serum level of IL-6 in baicalin treatment＋periodontitis group was significantly lower than that in periodontitis group at week 6 and 8（P〈0.01）.The serum level of IL-4 in periodontitis group was significantly lower than that in control and baicalin treatment＋periodontitis group（P〈0.01）.The serum level of IL-4 in baicalin treatment＋periodontitis group was significantly higher than that in periodontitis group at weeks 6 and 8（P〈0.01）.CONCLUSION： The pathogenesis of periodontitis is closely related to the imbalance of Th1/Th2 cytokines,characterized by increased serum level of IL-6 and the decreased serum level of IL-4.Baicalin plays a significant role in treating mouse periodontitis by decreasing the serum level of IL-6 and increasing the serum level of IL-4.