杧果种质遗传多样性的表型分析和AFLP分析

Genetic Diversity of Mango Germplasm Based on Morphological Charaters and AFLP Markers

利用表型和AFLP标记,对中国热带农业科学院南亚热带作物研究所杧果种质资源圃的56份国内外杧果种质进行遗传多样性分析。结果表明:这些种质的表型性状表现出较大的差异,多样性指数(H′)在0.56～1.64之间,平均为1.05,表型性状欧氏距离系数在0.02～0.45之间。8对AFLP引物共扩增出713条谱带,其中多态性带为630条,占88.36%,遗传相似性系数0.38～0.85之间。Mantal测量结果表明表型和基因型距离矩阵间存在显著的正相关(r=0.72,P=0.05)。表型性状和AFLP分子标记分析的结果相对一致,56份杧果种质具有较丰富的遗传多样性。

The genetic diversity of 56 accessions of mango germplasm,which were maintained in South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,China,were analyzed using morphological characters and AFLP markers. The results showed that these mango germplasm have rich genetic variation in morphology. The Shannon-Weinner index ranged from 0.56 to 1.64,and the Euclid distance of the morphological characters analyze ranged from 0.02 to 0.45. A total of 713 fragments were generated from 8 pairs of selective AFLP primer combination,630（88.36%）of which were polymorphic. The molecular genetic similarity coefficients ranged from 0.38 to 0.85. The Mental test showed that phenotypic variation was obviously positively correlated with the genetic distance based on AFLP markers（r = 0.72,P = 0.05）. Both our molecular and morphological data suggested that the mango germplasm were rich in genetic diversity.