贝那普利对肝纤维化大鼠ACE2和Ang-(1-7)的影响

Effect of Benazepril on ACE2 and Ang-(1-7) of hepatic fibrosis in rats

目的研究血管紧张素转化酶抑制剂(ACEI)贝那普利对大鼠肝纤维化模型的疗效及对肝脏血管紧张素转化酶2(ACE2)和血管紧张素-(1-7)[Ang-(1-7)]的影响。方法制备四氯化碳(CCl4)诱导的大鼠肝纤维化模型,同时应用贝那普利灌胃,共8周。测定各组门脉压力,对肝组织进行常规HE及Masson三色染色,免疫组织化学染色ACE2,酶联免疫法测定Ang-(1-7)水平。结果贝那普利可明显改善肝纤维化的程度,降低门脉压力(P<0.05),并可增加血浆Ang-(1-7)水平(P<0.01)。结论贝那普利具有良好的抗肝纤维化作用,可能与增加Ang-(1-7)有关。

Objective To explore the protective effects of angiotensin converting enzyme inhibitors benazepril on hepatic fibrosis induced by chronic carbon tetrachloride（CCl4） in rats and to observe the effect on angiotensin converting enzyme2（ACE2） and angiotensin-（1-7）[Ang-（1-7）] on the liver.Methods Except rats in control group,all were given intraperitoneal injections of 40 % CCl4,and from the first day of the intraperitoneal injection,rats in treatment group were given benazepril for 8 weeks by gastric gavage.Liver tissue and blood samples of all the rats were examined at the end of 8 weeks.Portal pressure was measured and histopathological study of the liver tissue was done with hematoxylin-eosin（HE） and Masson trichrome staining.ACE2 were evaluated by immunohistochemistry.Ang-（1-7） levels were determined by enzyme-linked immunosorbent assay（ELISA）.Results Benazepril significantly attenuated the degree of hepatic fibrosis,reduced portal pressure（P0.05） and elevated plasma Ang-（1-7） level（P0.05）.Conclusion Benazepril can delay the progression of hepatic fibrosis.It＇s probably relative to increase Ang-（1-7） level.