胃癌组织中CD133蛋白表达及其临床意义

Expression of CD133 Protein in Primary Lesions of Gastric Cancer and Its Clinical Significance

目的研究胃腺癌组织中CD133蛋白表达及其临床意义。方法应用免疫组织化学染色方法检测99例胃癌患者手术切除的原发灶及正常胃黏膜组织中CD133蛋白的表达,分析其与临床病理特征和预后的关系。结果 29例(29.29%)患者肿瘤组织中CD133蛋白表达阳性,正常胃黏膜组织中均为阴性(P=0.000)。肿瘤直径>5 cm者CD133蛋白表达阳性率显著高于≤5 cm者(P=0.041);CD133蛋白表达与TNM分期有关(P=0.044);有淋巴结转移(P=0.017)、淋巴管浸润(P=0.000)和血管浸润(P=0.000)者,CD133蛋白表达显著增高。logistic回归分析显示:肿瘤浸润深度(P=0.011)、淋巴结转移(P=0.043)和TNM分期(P=0.049)分别是CD133蛋白表达阳性的独立危险因素。CD133蛋白表达阳性患者的术后生存时间短于表达阴性患者(P=0.046)。Cox比例风险回归模型分析显示,有淋巴结转移(P=0.042)、TNM分期(P=0.046)及CD133蛋白表达阳性(P=0.046)分别是胃癌患者预后的独立危险因素。结论胃癌组织中CD133蛋白的表达与胃癌的发展、转移及预后密切相关。

Objective To investigate the expression of CD133 protein in primary lesions of gastric cancer and its clinical significance.Methods The expressions of CD133 protein in the primary lesion of tumor and normal gastric mucosa tissues confirmed by using histopathologic examination of 99 patients were detected by immunohistochemical staining.The correlation of CD133 protein expression with the clinicopathologic parameters and features after operation were analyzed.Results Positive cells of CD133 protein were localized in the gland parietal and cell membrane surface.The expression of CD133 protein in the cancer and normal gastric mucosa tissues were 29.29%（29/99） and zero,respectively（P=0.000）.Expression of CD133 protein in tumor with diameter 5 cm was significantly higher than that in the tumor with diameter ≤5 cm（P=0.041）.The expression of CD133 protein was correlated with TNM stage（P=0.044）,lymph node metastasis（P=0.017）,lymphatic vessel invasion（P=0.000）,and vascular invasion（P=0.000）.Logistic regression analysis revealed that invasion depth of tumor（P=0.011）,lymph node metastasis（P=0.043）,and TNM stage（P=0.049） were independent risk factors for CD133 protein expression.Survival time of patients with positive expression of CD133 protein was significantly shorter than that negative expression of CD133 protein（P=0.046）.Cox proportial hazard regression model analysis demonstrated that lymph node metastasis（P=0.042）,TNM stage（P=0.046）,and positive expression of CD133 protein（P=0.046） were independent risk factors for patients survival.Conclusion The CD133 protein expression in primary lesions is closely related with development,metastasis,and prognosis of gastric cancer.