脑梗死与对氧磷酶1基因192G/A位点多态性的关系

Relationship of cerebral infarction with paraoxonase 1 gene 192G/A polymorphism

目的:评估脑梗死患者对氧磷酶1(paraoxonase 1,PON1)的基因192G/A多态性与脑梗死间的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析295例脑梗死患者(脑梗死组)及117名正常对照者(对照组)的PON1基因192G/A多态性。结果:PON1基因192G/A多态性在对照组和脑梗死组中的GG型、GA型和AA型基因型分布分别为36.75%、50.43%、12.82%和28.14%、50.85%、21.02%。G、A等位基因分布在对照组和脑梗死组中分别为61.97%、38.03%和53.56%、46.44%。经检验,PON1基因192G/A位点基因型频率在2组人群中的分布差异无统计学意义(P>0.05),而等位基因频率在2组人群中的分布差异有统计学意义(P<0.05),脑梗死组的等位基因A与对照组比较,其比数比为1.2210,95%可信区间(95%CI)为1.0147～1.4793。结论:PON1基因192G/A多态性可能与脑梗死有关。

Objective To investigate the relationship of cerebral infarction with paraoxonase 1（PON1）gene 192G/A polymorphism.Methods Genotypes of PON1 gene 192G/A were determined in 295 cerebral infarction patients and 117 controls by using polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method.Results The distributions of GG,GA and AA genotypes of PON1 gene were 36.75%,50.43% and 12.82% in control group and 28.14%,50.85% and 21.02% in cerebral infarction group,respectively.The frequencies of allelic G and A were 61.97% and 38.03% in control group and 53.56% and 46.44% in cerebral infarction group,respectively.The difference of genotype frequency of PON1 192G/A was not statistically significant（P0.05） but that of allelic frequency was statistically significant（P0.05） between the two groups.Odds ratio of allele A in cerebral infarction group was 1.2210 with 95% confidence interval 1.0147-1.4793 as compared with the control group.Conclusions PON1 gene 192G/A polymorphism might have relation with cerebral infarction.