永磁磁场对胎鼠大脑皮质神经元细胞的影响

Influence of magnetic fields on fetal rat cortical neurons

目的以磁源空间磁场定量计算为依据,研究不同强度永磁磁场对胎鼠大脑皮质神经元细胞的影响。方法选取极面中心磁感应强度为3.9、8.0、12.1、20.5、27.0、80.0、170.0、400.0 mT的8组圆片磁源(即A、B、C、D、E、F、G、H组),采用有限元数值法计算其空间磁场,得出磁感应强度。取孕17d胎鼠大脑,分离皮质神经元细胞;将8种不同磁感应强度的磁源分别作用于大鼠脑皮质神经元细胞进行体外培养,6 d后分别用四甲基偶氮唑盐(MTT)法和乳酸脱氢酶(LDH)法检测细胞活力。设非加磁培养大鼠脑皮质神经元细胞为空白对照组。对各组大鼠脑皮质神经元细胞做统计学分析。结果各加磁组皮质神经元细胞光密度(OD)(A组1.204±0.003,B组1.210±0.003,C组1.202±0.004,D组1.220±0.001,E组1.172±0.002,F组1.223±0.012,G组1.235±0.010,H组1.028±0.060)均比空白对照组OD(1.275±0.002)低,其中A、B、C、D、E、H组与空白对照组比较,差异具有统计学意义(P<0.05或0.01),F、G组与空白对照组差异无统计学意义(P>0.05);各加磁组皮质神经元细胞上清液中LDH水平(A组13.33±0.33,B组12.33±0.33,C组8.33±2.33,D组10.67±2.33,E组11.33±4.33,F组10.00±1.00,G组9.00±0.30,H组9.67±0.33)均比空白对照组LDH水平(8.330±0.003)高,其中A、B、D、E、F、H组与空白对照组比较,差异具有统计学意义(P<0.05或0.01),C、G组与空白对照组差异无统计学意义(P>0.05)。结论实验所使用各磁感应强度组磁场对胎鼠大脑皮质神经元细胞代谢有一定抑制作用。

Objective To study the effect of permanent magnetic fields on fetal rat cortical neurons,and explore the dose-effect relationship based on the calculated intensities of space magnetic fields.Methods Eight kinds of circular magnets were selected,with the magnetic induction intensity at polar center of 0,3.9,8.0,12.1,20.5,27.0,80.0,170.0 and 400.0 mT（control group,A,B,C,D,E,F,G,H group）,respectively.The finite element method was used to calculate magnetic induction intensity of the magnetic space fields.Then,the magnets were used to treat the neurons from rat cerebral cortex in vitro,respectively.Six days after the treatment,the viability of the neurons were measured with [3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide] method and lactate dehydrogenase（LDH） method.Results The optical density（OD） of A,B,C,D,E,F,G,H group were 1.204 ± 0.003,1.210 ± 0.003,1.202 ± 0.004,1.220 ± 0.001,1.172 ± 0.002,1.223 ± 0.012,1.235 ± 0.010 and 1.028 ± 0.060 respectively.There were significantly different in A,B,C,D,E,H group and control group（1.275 ± 0.002）（P 0.05,0.01）;but not in F,G group and control group（P 0.05）.The LDH values of A,B,C,D,E,F,G,H group were 13.33 ± 0.33,12.33 ± 0.33,8.33 ± 2.33,10.67 ± 2.33,11.33 ± 4.33,10.00 ± 1.00,9.00 ± 0.30,9.67 ± 0.33.There were significantly different in A,B,D,E,F,H group and control group（8.330 ± 0.003）（P 0.05,0.01）;but not in C,G group and control group（P 0.05）.Conclusion It is demonstrated that magnetic fields have an inhibitiory effect on the growth of fetal rat cortical neurons in vitro.