PCNA在宫颈鳞癌组织中的表达及意义

Expression of PCNA protein in tissues of cervical squamous cell carcinomas and its clinical significance

目的探讨细胞核增值抗原(proliferating cell nuclear antigen,PCNA)在宫颈鳞癌和宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)组织中的表达及意义。方法采用免疫组织化学S-P法检测92例宫颈鳞癌、40例CIN和92例宫颈正常组织的PCNA蛋白表达情况。结果宫颈鳞癌组织PCNA阳性表达率为82.61%(76/92);CINⅠ、CINⅡ和CINⅢPCNA阳性表达率分别为33.33%(5/15)、71.43%(10/14)和81.82%(9/11),正常宫颈组织无PCNA表达。宫颈鳞癌、CINⅡ和CINⅢ组织PCNA阳性表达率明显高于CINⅠ(P<0.01);但宫颈鳞癌PCNA表达与CINⅡ、CINⅢ比较,差异无统计学意义(P>0.05)。PCNA阳性表达与宫颈癌分期(r=0.634,P<0.01)、组织学分级(r=0.654,P<0.05)有关;而与年龄无关(r=0,P>0.05)。结论 PCNA的阳性表达可能与宫颈癌的发生、发展及细胞增殖活性增加有关。

Objective To explore the protein expression of proliferating cell nuclear antigen（PCNA）in the tissues of cervical squamous cell carcinomas（CSCC）and cervical intraepithelial neoplasia（CIN）.MethodsPCNA expression were examined by S-P immunohistochemical stain（IHC）method in samples of both tumor and normal cervical tissue of 92 CSCC patients and 40 CIN tissues.ResultsThe rate of PCNA positive expression were 82.61%（76/92）in CSCC and 33.33%（5/15）,71.43%（10/14）,81.82%（9/11）in CINI,CINII and CINIII groups,respectively.The positive expression was not found in normal tissues.The protein expression of PCNA in CSCC,CINⅡ,CINⅢ groups,were higher than that in CINⅠgroup（P0.01）,but there were no significant difference between CSCC,CINⅡand CINⅢ groups（P0.05）.ConclusionsThe expression of PCNA was correlated with the progress,histological graduation and clinical stages of cervical cancer,but has no correlation with age.