脐血浆神经营养活性蛋白成分对CD34^+细胞增殖分化的影响

Effects of neurotrophic factors in umbilical cord blood plasma on CD34^+ cell proliferation and differentiation

背景:迄今为止,人们对于脐血浆中是否存在活性成分及其对于神经发育及神经损伤修复的作用认识尚不足,有待深入研究。目的:检测脐血浆生物活性成分,观察其在神经发育及神经损伤修复中的作用。方法:采用抗体芯片技术对脐血浆和健康青年女性静脉血浆活性成分进行对比分析,采用免疫磁珠从脐血中分选出CD34+细胞,经体外培养计数观察CD34+细胞增殖能力,通过免疫组化法观察细胞分化情况。结果与结论:脐血浆中有31种蛋白分子的含量显著高于静脉血浆,其中具有促进神经再生和神经修复潜能的活性蛋白10种,包括FGF4、Frizzled-3、IL-3、RAGE、CRIM-1、Neuritin、Neuropilin-2、Neurturin、SFRP-3、Tomoregulin-1;在CD34+细胞培养液中加入脐血浆能显著促进细胞增殖,促进细胞向神经细胞分化。

BACKGROUND：Further studies are needed to understand if the neurotrophic factors in human umbilical cord blood plasma have the promoting effect on neural regeneration and repair.OBJECTIVE：To investigate the component of neurotrophic factors in umbilical cord blood plasma,observe its application in neural regeneration and repair.METHODS：The difference was compared between the human umbilical cord blood plasma（HUCBP） and peripheral venous blood plasma（PVBP） from healthy voluntary womenfolk using human antibody array.The CD34＋ cells were sorted from human umbilical cord blood using immunomagnetic beads.Following cultivating in vitro and counting,proliferative ability of CD34＋ cells was observed.Cell differentiation was observed using immunohistochemical method.RESULTS AND CONCLUSION：The concentrations of 31 kinds of protein molecules were significantly higher in HUCBP than PVBP.Of them,10 kinds of molecules thereinto have significant potential of nerve regeneration and plasticity,including FGF4,Frizzled-3,IL-3,RAGE,CRIM-1,Neuritin,Neuropilin-2,Neurturin,SFRP-3 and Tomoregulin-1.HUCBP may promote the CD34＋ cells proliferation in cell culture medium and differentiation into nerve cells.