增龄因素与压力侧牙周组织Wnt-1及护骨素的表达

Age-related effect on Wnt-1 and osteoprotegerin expression in pressure side of periodontal tissue

背景:护骨素在正畸牙骨改建中具有重要作用,Wnt-1与骨形成密切相关,但与正畸骨改建的相关性未见报道。目的:比较增龄因素对大鼠正畸牙牙周组织Wnt-1及护骨素表达的影响。方法:纳入雄性6,24周龄Wistar大鼠各40只,制备大鼠磨牙向近中移动的正畸牙移动模型,于牙齿移动1,3,7,14d分别取材,采用RT-PCR及免疫组织化学方法检测压力侧牙周组织中Wnt-1及护骨素的表达。结果与结论:护骨素在6,24周龄大鼠压力侧牙周组织中均有表达,其中3,7d表达强于1,14d,相同时间点,6周龄大鼠压力侧护骨素的表达强于24周龄大鼠。6,24周龄大鼠压力侧牙周组织中均未见Wnt-1mRNA的表达,Wnt-1蛋白仅在6周龄大鼠牙齿移动3d时有表达。说明增龄因素是影响正畸骨改建的重要因素,而Wnt-1似乎与正畸骨改建无相关性。

BACKGROUND：Osteoprotegerin（OPG） plays an important role in bone remodeling during orthodontic tooth movement.Wnt-1 is closely related with bone formation,but Wnt-1 correlated with orthodontic bone remodeling has not been reported.OBJECTIVE：To compare age-related effect on periodontal tissue Wnt-1 during orthodontic tooth movement in rats and osteoprotegerin expression.METHODS：A total of 40 male Wistar rats（6-week-old） and 40 male Wistar rats（24-week-old） were included.Orthodontic tooth movement model of rat molar mesial movement was prepared,the model was drawn at 1,3,7,and 14 days tooth movement.The expression of Wnt-1 and OPG in pressure side of periodontal tissue was detected by RT-PCR and immunohistochemistry method.RESULTS AND CONCLUSION：OPG was expressed at the pressure side of periodontal tissues,which was stronger on days 3 and 7 than days 1 and 14;at the same time point,the OPG expression of pressure side of rats（6-week-old） was stronger than that of 24-week-old rats.Wnt-1 was not expressed at the pressure side of periodontal tissue in rats（6-week-old,24-week-old）.Wnt-1 protein expression of 6-week-old rats was existed at 3 days after tooth movement.Age factor is an important factor in orthodontic bone remodeling;however,Wnt-1 seems no correlation with orthodontic bone remodeling.