转化生长因子β1与体外血管外膜成纤维细胞的增殖和迁移

Transforming growth factor beta 1 on proliferation and migration of adventitia fibroblasts in vitro

背景:近年来的研究表明,血管外膜成纤维细胞在血管损伤后新生内膜的增生中起重要作用。当血管损伤后,转化生长因子β1在局部水平上调激活下游多种信号途径,其对血管外膜成纤维细胞参与新生内膜增生过程中所起的作用尚不十分清楚。目的:观察转化生长因子β1对体外血管外膜成纤维细胞增殖和迁移的影响方法:体外培养大鼠胸主动脉成纤维细胞,分别以0,3,5,10,15μg/L的转化生长因子β1刺激成纤维细胞0,2,12,24,48,72h。以MTT法检测细胞增殖活性,Transwell Chamber测试细胞迁移。结果与结论:转化生长因子β1能诱导血管外膜成纤维细胞增殖,随着转化生长因子β1质量浓度、作用时间的增加,诱导血管外膜成纤维细胞增殖能力增强(P<0.05),其中10μg/L转化生长因子β1作用48h增殖较对照组增强最为明显(P<0.01)。不同剂量转化生长因子β1刺激后迁移的细胞数目随转化生长因子β1质量浓度增加而增加,促进作用逐渐增强,呈剂量依赖关系。

BACKGROUND：Previous studies demonstrated that adventitia fibroblasts exhibit important role in the hyperplasia of newly born endomembrane after blood vessel injury.Transforming growth factor β1（TGF-β1） can stimulate many signways after blood vessel injury.However,the effect of TGF-β1 on proliferation and migration of adventitia fibroblasts in vitro remains unclear.OBJECTIVE：To observe the effect of TGF-β1 on proliferation and migration of adventitia fibroblasts in vitro.METHODS：Fibroblasts from rat thoracic aorta were in vitro cultured and stimulated by TGF-β1 with different concentrations（0,3,5,10 and 15 μg/L） for different hours（0,2,12,24,48,and 72 hours）.MTT assay were applied to assess cellular proliferation.Migration assay was performed by transwell chambers.RESULTS AND CONCLUSION：TGF-β1 could potentiate the proliferation of adventitia fibroblasts,and the effects were increased with the increasing of TGF-β1 concentrations and TGF-β1 inducing times（P 0.05）,especially stronger in the group treated by 10 μg/L TGF-β1 for 48 hours（P 0.01）.The migration of adventitia fibroblasts were increased with the increasing of TGF-β1 concentrations in a dose-dependent fashion.