脊髓全横断大鼠模型的构建

Establishment of spinal cord transection injury models in rats

背景:脊髓全横断模型在造模时常难以保证神经纤维的完全离断。目的:构建大鼠脊髓全横断损伤模型。方法:将大鼠随机分为模型组和假手术组。模型组构建脊髓T10节段全横断模型;假手术组动物仅打开椎管与硬脊膜而后缝合,但不损伤脊髓。建模后1,3,5,7d分别进行BBB评分以评估后肢运动功能,检测其体感诱发电位和运动诱发电位来评估神经传导通路的完整性,并行形态学观察来评估脊髓肉眼观病理形态。结果与结论:与假手术组相比,模型组大鼠在建模后1,3,5,7d时,其BBB评分降低(P<0.01),未检测出体感和运动诱发电位。形态学观察结果显示模型组大鼠脊髓完全横断,而假手术组脊髓形态完整。结果提示实验成功构建了大鼠脊髓全横断模型。

BACKGROUND：The spinal cord injury（SCI） models reported in literature were short of determinacy and reliability.OBJECTIVE：To establish an accurate and reliable rat spinal cord transection injury models.METHODS：Rats were divided into model and sham-surgery groups at random.Model group prepared for T10 transection injury models;vertebral canal and spinal dura mater were exposed and sutured in the sham-surgery group.The motor function of hind limbs was evaluated by Basso,Beattie and Bresnahan（BBB） scores;the integrality of the nervous pathways was detected by somato-sensory evoked potential（SEP） and motor evoked potential（MEP）,in addition,the pathologic and gross pathology of spinal cord were performed at 1,3,5 and 7 days after modeling.RESULTS AND CONCLUSION：Compared with the sham-surgery group,the BBB scores of the model group were decreased at 1,3,5 and 7 days after modeling（P 0.01）.The SEP and MEP were not evoked out in the model group.The spinal cords were fully transected in the model group,which was intact in the sham-surgery group.that of.The result suggested that a spinal cord transection injury model is established successfully.