磷脂多不饱和脂肪酸抑制兔耳增生性瘢痕

Phospholipid polyunsaturated fatty acid inhibits hypertrophic scar in rabbit ears

背景:多不饱和脂肪酸有抑制细胞炎症反应及免疫功能的作用,增生性瘢痕的形成与炎症、细胞免疫、细胞因子有着密切关系,但目前尚无应用多不饱和脂肪酸防治增生性瘢痕的实验研究。目的:探讨磷脂多不饱和脂肪酸对兔耳增生性瘢痕的抑制作用。方法:在9只新西兰大白兔兔耳腹侧做直径1cm的圆形全层皮肤缺损创面,每侧6个,共108个,其中形成增生性瘢痕92个,瘢痕形成率为85%。实验分3组:每只兔耳靠前3个创面涂磷脂多不饱和脂肪酸霜,右耳靠后3个创面涂多磺酸黏多糖乳膏,创面上皮化后立即涂药,每日1次,左耳靠后3个创面自然愈合。分别在术后28,42,63,90d,观察创面的愈合情况;显微镜下观察瘢痕组织的厚度、胶原纤维和成纤维细胞密度;免疫组织化学染色检测胶原纤维的表达。结果与结论:涂抹磷脂多不饱和脂肪酸霜和多磺酸黏多糖乳膏可使增生性瘢痕体积缩小、厚度变薄、成纤维细胞密度减小、胶原纤维表达减少。尤以磷脂多不饱和脂肪酸霜的效果最为明显。说明磷脂多不饱和脂肪酸可抑制兔耳增生性瘢痕的形成,减轻瘢痕的增生程度。

BACKGROUND：Phospholipid polyunsaturated fatty acid（Phospholipid-PUFA） can inhibit the cell inflammatory response and immune function.The formation of hypertrophic scars has contact with inflammation,cellular immunity and cytokines closely.However,there are few studies addressing Phospholipid-PUFA on hypertrophic scars.OBJECTIVE：To explore the inhibiting effect of phospholipid-PUFA on rabbit ear hypertrophic scar.METHODS：Nine New Zealand rabbits were chosen to establish animal models of hypertrophic scars.Six round full-thickness wounds in sizes of 1 cm×1 cm were made in the ventral surface of each ear.Totally 108 wound were prepared,and formed 92 hypertrophic scars,with scar formation rate of 85%.Every 3 wounds in each front ear were treated by phospholipid-PUFA cream,those at the behind of right ear was treated by mult-acid mucopolysaccharide cream,once per day,the rest served as control.The wound healing was observed at 28,42,63 and 90 days after operation.The thickness of scars,collagen fiber and density of fibroblasts were observed under a microscope.The expression of collagen fiber was detected by immunohistochemistry.RESULTS AND CONCLUSION：Compared with the control group,the volume of hypertrophic scar was minificated,thinner,the density of fibroblasts was diminished,and the expression of collagen fiber was decreased in the experimental groups,especially obviously in the phospholipid-PUFA group,suggesting phospholipid-PUFA can inhibit hypertrophic scar formation and reduce scar hyperplasia.