Bcl-xL基因在正常皮肤血管组织、增生及退化期血管瘤组织中的表达

Expression of Bcl-xL gene in normal skin and the proliferating and degenerating hemangioma tissues

背景:有文献报道Bcl-xL基因能抑制细胞凋亡,并可能参与血管形成,但目前Bcl-xL基因在恶性肿瘤中的研究较多,而在血管瘤的研究较少。目的:应用免疫组织化学染色法检测增生期和退化期血管瘤和正常皮肤血管组织中Bcl-xL基因的表达。方法:收集有完整临床和病理资料的40例皮肤血管瘤手术切除标本,包括增生期血管瘤22例,退化期血管瘤18例。另取瘤组织周围正常皮肤组织5例作为对照。采用免疫组织化学染色方法检测Bcl-xL在各组中的表达,并结合Ⅷ因子相关抗原的免疫组织化学染色证实表达Bcl-xL阳性表达的细胞为血管瘤组织中的内皮细胞,并测定Bcl-xL在各组中的平均吸光度和平均阳性面积率。结果与结论:增生期血管瘤内皮细胞胞浆内可见较多的棕黄色颗粒沉积,Bcl-xL表达呈强阳性;退化期血管瘤和正常皮肤组织内皮细胞胞浆内可见少量的棕黄色颗粒或无棕黄色颗粒,Bcl-xL表达弱或无表达。增生期血管瘤与退化期血管瘤和正常皮肤组相比,Bcl-xL阳性表达的平均吸光度和阳性面积率明显增高(P<0.01)。结果显示,Bcl-xL为抗凋亡基因,在增生期时呈高表达,其促进了血管内皮细胞的增殖,参与了血管瘤的增生。

BACKGROUND：Bcl-xl gene can suppress cell apoptosis and may participate in angiopoiesis.However,more studies concerning Bcl-xL gene in malignant tumor,few are reported in hemangioma.OBJECTIVE：To study the expression of Bcl-xL gene in normal skin vessels,hyperplasia and degeneration hemangioma tissues using immune-histochemical technique.METHODS：Specimens were obtained from 40 cases of dermal hemangioma by exairesis,comprising 22 cases with proliferating hemangiomas and 18 with degenerating hemangiomas.In addition,normal skin tissues around hemangiomas from 5 cases were also chosen for controls.Immunohistochemical stainings were performed to detect the expression of Bcl-xL in those three groups.Endothelial cells were identified by expressing factor Ⅷ-related antigen.The average absorbance and the rate of positive area of expression of Bcl-xL were analyzed.RESULTS AND CONCLUSION：There were plenty of brown particles in the cytoplasm of proliferating endothelial cells,which strongly positive expressed Bcl-xL.There were few and mo brown particles in endothelial cells cytoplasm of the degenerating hemangiomas or normal skins.The expression of Bcl-xL were weakly and no expression.Compared with the normal skins,the average absorbance and rate of Bcl-xL positive expression were higher in the proliferative and degenerating hemangiomas（P 0.01）.Bcl-xL is an anti-apoptosis gene and was highly expressed in the proliferative phase,which promoted proliferation of blood vessel endothelial cells and participated in hyperplasia of hemangiomas.