人羊膜上皮细胞对大鼠坐骨神经再生影响的实验研究

The effect of human anmiotic epithelial cells on regeneration of rat sciatic nerve： an experimental study

目的通过实验研究了解人羊膜上皮细胞（human amniotic epithelial cells，HAECs）对大鼠坐骨神经再生的促进作用。方法取60只SD大鼠随机分为两组，羊膜细胞组和对照组，各组再分为2周和4周组，每组15只。制作大鼠周围神经损伤再生室模型，HAECs组局部应用培养的HAECs，对照组局部使用等量的生理盐水。术后分别于2周、4周检测神经传导功能和HE染色观察神经纤维形态学变化。结果术后2周两组的神经电生理及组织学检测比较差异无统计学意义；4周HAECs组的大鼠坐骨神经传导功能明显恢复，HE染色显示术后坐骨神经手术部位出现大量炎性肉芽组织，呈现纤维性修复，吻合口以远HAECs组神经纤维形态结构较对照组完整，神经纤维与髓鞘直径均较对照组大。结论HAECs移植可加速大鼠坐骨神经损伤后神经传导功能恢复，有助于有髓神经纤维损伤后的轴突与髓鞘的再生修复。

Objective To study the effects of hmnan anmiotic epithelial cells （HAECs） on regeneration of rat sciatic nerve. Methods Sixty SD rats were randomly divided into two groups, the HAECs group and control group. Each group was further divided into two week group and four week group, with 15 rats each. The sciatic nerve was cut and the regeneration chamber was created. The HAECs were implanted into the regeneration chamber in the HAECs group and normal saline was injected into the regeneration chamber in the control group. Electro-physiological and the histological study was done at two weeks and four weeks after the surgery. The results of nerve conduction study and HE staining of regenerating nerve fibers were analyzed statistically. Results There were no statistically significant differences between the two groups at two weeks post-operatively. At four weeks, the experimental group showed significantly higher nerve conduction velocity and amplitude of evoked potentials comparing to those of the control group. Nerve fibers and inflammatory granulation tissue was seen near the lesion site by HE staining in the control group, whereas the HAECs group showed more integrated nerve fibers and mature myelination distal to the lesion site. Conehlsion The application of human amniotic epithelial cells may enhance nerve regeneration in rats.