HPLC法检测人血浆中利奈唑胺浓度

Determination of linezolid in human plasma by HPLC

目的建立检测人血浆利奈唑胺的HPLC法。方法以Eclipse XDB-C_(18)为色谱柱;甲醇-水(45:55,V/V)为流动相,流速1.0mL·min^(-1);血浆样品经70%高氯酸沉淀后,在254nm波长下进行检测。结果利奈唑胺在质量浓度0.25～50mg·L^(-1)内线性关系良好(r=0.999 8),定量下限为0.25mg·L^(-1);低、中、高浓度的日间、日内精密度(RSD)均<10%,相对回收率分别为96.40%、103.24%、100.33%,提取回收率>75%。结论本方法操作简便,结果准确,适用于血浆中利奈唑胺浓度的检测。

AIM To establish a HPLC method for the determination of linezolid in human plasma.METHODS The analytical column was packed with Eclipse XDB-C_(18),the mobile phase was methanol-water(45:55,V/V),the flow rate was 1.0 mL·min^(-1),and the detection wavelength was 254 nm.Proteins in plasma samples were precipitated by 70%perchloric acid,and detected at 254 nm.RESULTS The liner range of linezolid in human plasma was 0.25-50 mg·L^(-1)(r =0.999 8).The limit of detection was 0.25 mg·L^(-1).The intra-day and inter-day RSD for assaying the plasma sample containing three concentration of linezolid were both lower than 10%.The relative recovery were 96.40%, 103.24%and 100.33%,respectively.The absolute recovery were more than 75%.CONCLUSION The HPLC method to determine linezolid in plasma is simple,high selectivity and sensitivity.