Rac1在人神经胶质瘤组织中的表达及其与病理分级的相关性研究

Relationship between Racl expression of human gliomas and their pathological grades

目的观察Rac1mRNA及蛋白在神经胶质瘤组织中的表达情况，探讨Rac1与病理分级的相关性。方法应用免疫荧光组织化学、RT。PCR和Western blotting方法检测45例神经胶质瘤组织和10例正常晒组织标本中Rac1 mRNA及蛋白水平。结果3种检测结果均表明，Rac1在正常脑组织中无表达，45例脑胶质瘤中最多42例有Rac1表达，表达率与病理分级呈正相关，即脑胶质瘤病理级别越高，Rac1阳性表达率越高。结论Rac1在脑胶质瘤中的高表达与神经胶质瘤的侵袭、转移密切相关，可做为一个反映脑胶质瘤增殖能力和恶性程度的指标。

Objective To investigate the protein and mRNA levels of Racl in glioma tissues, and explore the correlation of Racl with pathological grades. Methods Immunofluoreseence, RT-PCR and Western blotting were used to detect the protein and mRNA levels of Rac 1 in 45 cases of gliomas tissues and l0 cases of normal brain tissues. Results The results indicated that normal brain tissues showed no protein and mRNA expressions of Racl, and that of Rael highly expressed in glioma tissues （42/45 at most）. The spearman correlation analysis revealed that the levels of transcription and expression of Racl were positively correlated to the tumor grades; positive expression rate of Racl in high grade of glioma was statistically higher than that in low grade of glioma. Conclusion The high expressions of Racl in the glioma are closely correlated to the tumor cell invasion and metastasis, which can be used as a marker indicating the malignance and proliferation of glioma.