茶多酚对血管紧张素Ⅱ诱导的血管内皮细胞游离钙离子浓度的调节作用

Regulating effect of tea polyphenols on angiotensin-Ⅱ-induced calcium concentration in vascular endothelial cell

目的研究茶多酚(TPs)对血管紧张素Ⅱ(Ang-Ⅱ)诱导的血管内皮细胞(VEC)游离钙离子浓度([Ca2+]i)的调节作用。方法将VEC分为四组:Ang-Ⅱ10-7mol/L组(A组)、Ang-Ⅱ10-7mol/L+TPs 5 mg/L组(B1组)、Ang-Ⅱ10-7mol/L+TPs 25 mg/L组(B2组)及空白对照组(C组)。用激光共聚焦显微镜测量各组细胞的[Ca2+]i。结果与C组相比,A组[Ca2+]i增高(P<0.01);而B1、B2组[Ca2+]i明显低于A组(P<0.01),且B2组[Ca2+]i低于B1组(P<0.01)。结论 TPs对Ang-Ⅱ诱导的[Ca2+]i增加具有剂量依赖性的保护作用。

Objective To investigate the regulating effect of tea polyphenols（TPs） on angiotensin-Ⅱ（Ang-Ⅱ）-induced calcium concentration（／i） in vascular endothelial cells（VECs）.Methods The VECs were divided into four groups of A（Ang-Ⅱ 10-7mol/L）,B1（Ang-Ⅱ 10-7mol/L＋TPs 5 mg/L）,B2（Ang-Ⅱ 10-7mol/L＋TPs 25 mg/L） and C（normal control）.Intracellular ／i was detected by laser confocal microscopy.Results Compared with group C,／i was increased in group A（P0.01）.／i in groups of B1 and B2 was lower than that in group A（P0.01）.／i in group B2 was lower than that in group B1（P0.01）.Conclusion TPs can dose-dependently protect the vascular endothelial cell injury induced by Ang-Ⅱ-associated ／i increase.