乙肝标志物模式与病毒载量的相关性研究

Relationship between Viral Load and HBV Serum Markers

目的研究慢性乙肝患者血清标志物和病毒载量之间相关性。方法血清HBV DNA测定用荧光定量分析PCR法和乙肝标志物采用化学发光酶免疫定量法,分别对222例慢性乙肝患者血清HBV DNA和乙肝标志物检测并比对分析。结果血清学检测乙肝表面抗原(HBsAg)+乙肝病毒e抗原(HBeAg)+乙肝病毒核心抗体(HBcAb)组与乙肝表面抗原(HBsAg)+乙肝病毒e抗原(HBeAg)组其病毒载量显著高于其它组(病毒载量高于105拷贝/m l分别达91.9%和77.8%)。结论 HbeAg与HBV DNA定量水平具有良好的相关性,同时检测乙肝标志物和HBV DNA才能准确反映不同个体HBV感染状态及病毒复制情况。

Objective To explore the relationship between the serum HBV viral load and HBV serum markers in patients with hepatitis.Methods The serum levels of HBV-DNA copies and HBV serum markers 222 patients with chronic hepatitis B were examined and analyzed.HBV-DNA copies were detected by fluorescence quantitative PCR（FQ-PCR） and HBV serum markers by chemiluminescent immunoassay.The paired analysis was carried out.Results The serum contents of HBV viral load group A（HBsAg＋,HBeAg＋,HBcAb＋） and group D（HBsAg＋,HBeAg＋） was higher than that in other groups.High-levels viremia（HBV viral load〉105 copies/ml） was found both in group A and group D（91.9%,77.8%）.Conclusion There was a positive good correlation between the level of serum HBV-DNA and HBeAg content.Simultaneous determination of serum HBV viral load and HBV serum markers is necessary in determining the HBV infection and viral replication.