摘要
Nanographene oxide (NGO) is currently being explored for various biomedical applications.However,little information is known about its biological behaviors in vitro and in vivo.For further studying its pharmacokinetics and related biological behaviors in living systems,an effective and convenient tracing method is particularly demanded.In this work,NGO was labeled with radionuclide 188Re (188Re-NGO).To obtain high labeling yield and purity,a number of labeling conditions,including concentration of SnCl2 and ascorbic acid,reaction time and temperature,and pH were optimized,and stability of the 188Re-NGO in vitro and in vivo was evaluated.The results showed that NGO could be effectively labeled with high yield.The purified 188Re-NGO showed high stability in vitro and in vivo.A pretest of NGO biodistribution with single photon emission computed tomography showed that the 188Re-NGO was rapidly taken by organs such as lungs,liver,and spleen.The biodistribution of 188Re-NGO differs significantly from the free radionuclide,indicating that the labeling procedure is highly suitable for investigating its biological behavior in living systems.
Nanographene oxide （NGO） is currently being explored for various biomedical applications. However, little information is known about its biological behaviors in vitro and in vivo. For further studying its pharmacokinetics and related biological behaviors in living systems, an effective and convenient tracing method is particularly demanded. In this work, NGO was labeled with radionuclide ^188Re （^188Re-NGO）. To obtain high labeling yield and purity, a number of labeling conditions, including concentration of SnCl2 and ascorbic acid, reaction time and temperature, and pH were optimized, and stability of the ^188Re-NGO in vitro and in vivo was evaluated. The results showed that NGO could be effectively labeled with high yield. The purified ^188Re-NGO showed high stability in vitro and in vivo. A pretest of NGO biodistribution with single photon emission computed tomography showed that the ^188Re-NGO was rapidly taken by organs such as lungs, liver, and spleen. The biodistribution of ^188Re-NGO differs significantly from the free radionuclide, indicating that the labeling procedure is highly suitable for investigating its biological behavior in living systems.
基金
Supported by the National Science Foundation of China(No.10775169,10905086,10975179)
the Shanghai Municipal Natural Science Foundation(No.08ZR1422700,08JC1422600)
the Ministry of Health(No.2009ZX10004-301)
the CAS Innovation Program(No.095501K)
the MOST973Program(No.2006CB705605)
