白藜芦醇上调糖尿病大鼠脂肪组织PPAR-γ及GluT-4蛋白表达

Resveratrol Improving Insulin Sensitivity of Diabetic Rats by Increasing Protein Expression Levels of PPAR-γ and GluT-4 in Adipose Tissue

目的探讨白藜芦醇改善糖尿病大鼠脂肪组织胰岛素敏感性及降血糖的作用机制。方法雄性W istar大鼠随机分为对照组(NC,n=15)及糖尿病造模组(DM,n=40),DM组成模后随机分为糖尿病模型组(DM-C,n=15)和糖尿病白藜芦醇治疗组(DM-T,n=15)。DM-T组予白藜芦醇5.0 mg/(kg·d)-1灌胃11周。实验末,检测各组大鼠FBG、FIns、TG、TC、胰岛素敏感指数(ISI)、过氧化物酶体增殖物激活受体γ(PPAR-γ)及葡萄糖转运蛋白4(GluT-4)在脂肪组织的表达水平。结果 17周末,DM-C组较NC组FBG、TG明显升高,ISI明显降低,脂肪组织PPAR-γ及GluT-4蛋白表达明显降低;DM-T组较DM-C组FBG明显降低;ISI明显升高;PPAR-γ和GluT-4蛋白表达明显升高。结论白藜芦醇可上调糖尿病大鼠脂肪组织PPAR-γ及GluT-4蛋白的表达,降血糖并改善胰岛素敏感性。

Objective To investigate the mechanism for resveratrol to improve the insulin sensitivity in diabetes mellitus.Methods Male Wistar rats were randomly divided into normal control group（NC,n=15） and diabetic group（DM,n=40） induced by STZ and high-fat diet.Diabetic rats were randomly divided into diabetic control group（DM-C,n=15） and diabetic group treated with resveratrol（DM-T,n=15）.Then DM-T group was given intragastric administration with resveratrol at the dose of 50 mg/（kg·d）-1 for 11 weeks.FBG,fasting serum insulin（FIns）,triglyceride（TG）,total cholesterol（TC）,insulin sensitivity index（ISI） and other biochemical parameters in each group were measured.The protein expressions of PPAR-γ and GluT-4 were determined by Western blot..Results At the end of the 17th week,compared with NC group,DM-C group showed higher FBG and TG,lower ISI,lower protein expressions of PPAR-γ and GluT-4 in the adipose tissue.Compared with DM-C group,DM-T group showed lower FBG,higher ISI.Integrated density（ID） showed higher protein expression of PPAR-γ of DM-T group in adipose tissue as compared with DM-C group.Integrated density（ID） showed higher protein expression of GluT-4 of DM-T group in adipose tissue as compared with DM-C group.Conclusion Resveratrol could increase the protein expressions of PPAR-γ and GluT-4 in adipose tissue,decreases FBG and improves insulin sensitivity of diabetic rats.