ZMS对老年大鼠脑M受体与G-蛋白偶联活性的影响

The effect of ZMS on the coupling of muscarinic receptor to G-proteins activation in rat brain

以非ＧＴＰａｓｅ水解性的［３５Ｓ］ＧＴＰγＳ在激动剂Ｃａｒｂａｃｈｏｌ作用下和Ｇ－蛋白不可逆结合，检测大鼠脑Ｍ受体与Ｇ－蛋白偶联活性，同时观察不同剂量ＺＭＳ对其活性和学习记忆的影响。结果老年组Ｇ－蛋白活性低于青年组，中、大剂量组优于老年组，并能改善其学习记忆能力。Ｔａｃｒｉｎｅ无明显作用。提示老年大鼠Ｍ受体与Ｇ－蛋白偶联可能有障碍，ＺＭＳ对大鼠学习、记忆的促进作用可能与提高Ｍ受体与Ｇ－蛋白偶联活性有关。

The carbachol-stimulated [~35S]GTP r S binding method was used to observe theeffect of ZMS, an active component from Zhimu, on the coupling of M-receptor toG-protein. The effect of ZMS on the ability of learning and memory in aged rats wasalso observed. It was shown that the carbachol-stimulated elevation of [~35S]GTP r Sbinding was significantly decreased in aged rats as compared with young rats. Thecarbachol-induced [~35S]GTPr S binding showed that administration of ZMS atmedian or high dose have a definite elevation effect on the coupling activity ofM-receptors to G-protein in brain, and this elevation was accompanied by animprovement of learning and memory ability.