原发性乳腺癌SSTR1-3mRNA分布特性的研究

Studies on mRNA expression of the somatostatin receptor family in breast cancer

为研究生长抑素受体三种亚型（ＳＳＴＲ１－３）在原发性乳腺癌中的分布特性，以［０６３３α－３５Ｓ］ｄＡＴＰ标记ＳＳＴＲ三种亚型的寡核昔酸为探针，运用原位杂交技术，检测１０例乳腺癌组织中ＳＳＴＲ１—３ｍＲＮＡ的表达模式，并用Ｌｅｉｃａ图像分析仪对表达密度作半定量分析。结果表明，ＳＳＴＲ１—３在乳腺癌中的表达模式以表达ＳＳＴＲ２为主（７／１０）；从ＳＳＴＲ１—３在乳腺癌中的表达密度看，ＳＳＴＲ２高于其它两种亚型（８２．３±１．４）；与ＳＳＴＲ１、３相比，ＳＳＴＲ２为优势表达亚型（Ｐ＜０．０１）．说明ＳＳＴＲ３种亚型在乳腺癌中可同时表达，其分布模式呈异质性，其表达模式存在着较大的个体差异，从表达率及表达密度的半定量分析来看，ＳＳＴＲ２均为主要表达亚型，提示乳腺癌具有接受生长抑素类似物靶向药物治疗的乐观前景。

The objective of this paper is to investigate the characteristics of expression anddistribution of 3 subtypes of somatostatin receptors (SSTRI-3) in breast cancer.With [a-~35S] dATP labeled oligonucleotide of the 3 SSTR subtypes as probe, using insitu hybridization, patterns of mRNA expression were detected in 10 breast cancercases. Additionally, semi-quantitative analysis of the densities of the expression wereestimated by a compreterized image-analysis system. Patterns of SSTR1-3expression in breast cancer were as follows: SSTR2 expression was dominant in 10cases than that of the other 2 subtypes; Therefore, it has positive prospects forsomatostatin analog-oriented agents to be used in treatment of breast cancers.