拮抗真菌菌株BOS-013对稻瘟病菌的抑制作用

Inhibition Effect of Antagonistic Fungal Strain BOS-013 against the Pathogen of Rice Blast(Pyricularia oryzae)

[目的]为寻找对水稻稻瘟病的有防治作用的有益微生物及其代谢产物,研究放线菌BOS-013菌株发酵液对水稻稻瘟病菌的抑制作用。[方法]以水稻稻瘟病菌作为目标指示菌,采用杯碟法和滤纸片法测定BOS-013菌株发酵液及其不同溶剂提取物的抑菌活性。[结果]研究结果表明,含菌量为4×108 cfu/ml的BOS-013菌株发酵液对水稻稻瘟病菌的抑制率为92.0%±0.5%;该发酵液经高温、高压灭活后对水稻稻瘟病菌仍有抑制作用,抑制率为60.0%±0.5%。发酵液经非极性CAD-45型大孔吸附树脂吸附、60%乙醇溶液洗脱后,其抑菌活性很高,抑菌圈直径达33.0 mm。[结论]BOS-013菌株发酵液水稻稻瘟病菌有显著的抑制作用;该研究结果为进一步明确BOS-013菌株发酵液对水稻稻瘟病菌的抑制作用机理及其实际应用奠定了基础。

[Objective] To search for the beneficial microorganisms and their metabolic products with control effect against rice blast,the paper studied the inhibition effect of the fermentation broth of actinomycetes BOS-013 strain against the pathogen of rice blast（Pyricularia oryzae）.[Method] With the pathogen of rice blast as the target indicator fungus,the inhibition effect of the fermentation broth of BOS-013 strain and its extracts from different solvents against the pathogen were determined using cylinder plate method and filter paper method.[Result] The results showed that the inhibition rate of the fermentation broth of BOS-013 strain with fungus amount of 4×108 cfu/ml against the pathogen of rice blast was 92.0%±0.5%;the fermentation broth still had inhibition effect against the pathogen of rice blast after inactivation under high temperature and high pressure,and the inhibition rate was 60.0%±0.5%.After absorbed by non-polar CAD-45 type macroporous adsorption resin and eluted by 60% ethanol,the antifungal activity of the fermentation broth was still high,and the diameter of inhibition zone reached 33.0 mm.[Conclusion] The fermentation broth of BOS-013 strain had significant inhibition effect against the pathogen of rice blast.The results would provide foundation for further clarification of inhibition mechanism of BOS-013 strain fermentation broth against the pathogen of rice blast and its practical application.