不同秸秆沼气发酵高效产酸复合菌系的筛选

Screening of Efficient Acid-production Composite Microbial System in the Biogas Fermentation of Different Straw

[目的]在不同秸秆的沼气发酵中筛选高效产酸复合菌系。[方法]以麦秆、玉米秆为碳源,牛粪为氮源,研究不同菌群经多代训化后,以不同秸秆为碳源时的产酸情况,并采用蒸馏法测定培养液中总挥发性脂肪酸的含量。[结果]以麦秆为碳源时,来自牛胃内容物的菌群具有高效产酸能力;以玉米秸秆和混合秸秆为碳源时,来自腐殖土的菌群具有高效产酸能力。[结论]该研究有助于提高两相沼气生产中产酸相秸秆木质素的利用率和产气相的产气率。

[Objective] The research aimed to screen the efficient composite microbial system which produced the acid in the biogas fermentation of different straw.[Method] The wheat straw and corn straw were the carbon sources,and the cow dropping was the nitrogen source.After the multi-generations training,the acid production situations of different microbial systems when the different straw was the carbon source were studied.Moreover,the distillation was used to measure the content of total volatile fatty acid（VFA）.[Result] When the wheat straw was the carbon source,the microbial system from the bovine stomach content had the efficient acid-production ability.When the corn straw and the mixed straw were the carbon sources,the microbial system from the humus soil had the efficient acid-production ability.[Conclusion] The research was favorable to improve the utilization ratio of straw lignin in the acidogenic phase and the gas-production rate of gas-production phase in two-phase biogas production.