大蒜素对兔急性下肢缺血再灌注损伤后组织中IL-1,IL-6,IL-8含量的影响

The impact of allicin on rabbits with acute limb ischemia reperfusion tissue by IL-1,IL-6,IL-8 content

目的 观察大蒜素注射液对兔急性下肢缺血再灌注损伤后组织中白介素-1、白介素-6、白介素-8含量的影响及意义.方法 30只家兔随机分为5组：空白组、缺血再灌注2h组、缺血再灌注5h组、缺血再灌注2h大蒜素治疗组、缺血再灌注5h大蒜素治疗组.复制缺血再灌注模型.空白组,切开下肢,点滴生理盐水,取腓肠肌.其余四组于缺血2h或5h结束前10min,分别于耳缘静脉点滴大蒜素或生理盐水,再灌注1h后取腓肠肌.分别作免疫指标测定和形态学观察,进行对比分析.结果 缺血再灌注2h组、5h组组织中IL-1,IL-6,IL-8含量与空白组比较有明显增高（P〈0.05）,而缺血再灌注2h大蒜素治疗组、缺血再灌注5h大蒜素治疗组分别与缺血再灌注2h组、缺血再灌注5h组比较IL-1、IL-6、IL-8含量明显下降（P〈0.05）.光镜观察,再灌注组组织结构改变明显,而治疗组改变较轻.结论 大蒜素在兔急性下肢缺血再灌注损伤中能抑制IL-1,IL-6,IL-8的表达,有效减少中性粒细胞的黏附、浸润,减轻炎性渗出,押制微循环通透性的增加,减轻下肢肌肉的损伤.

Objective To observe the impact and significance of allicin injection on rabbits with acute limb ischemia reperfusion tissue by IL-I,IL-6,IL-8 content. Methods 30 rabbits were randomly divided into 5 groups：control group, ischemia-reperfusion 2 hours group, ischemia-reperfusion 5 hours group, ischemia- reperfusion 2 hours with allicin treatment group, ischemia-reperfusion 5 hours with aUicin treatment group. Copying ischemia-reperfusion model in the following way. Control group, cutting limbs with saline drops and taking gastrocnemius.The remaining four groups：we made allicine or saline drops in the ear vein 10 minutes before the deadline of ischemia 2 or 5 hours groups,taking gastrocnemius after ischemia-reperfusion for 1 hour as immune parameters measurement and comparative analysis of morphological observation. Results IL-I,IL-6,IL-8 content in tissues of ischemia-reperfusion 2 hours group and 5 hours group were significantly higher than that of control group （P 〈0.05）. IL-I,IL-6,IL-8 content in tissues of ischemia- reperfusion 2 hours with allicin treatment group and ischemia-reperfusion 5 hours with aUicin treatment group were significantly lower than that of ischemia-reperfusion 2 hours group and 5 hours group （P 〈 0.05）.The physiological changes of tissues structure was much more obvious with both the ischemia-reper- fusion group but less obvious with both the treatment group at light microscope.Conclusion Allicin may in- hibit the expression of IL-I,IL-6,IL-8 on rabbits with acute limb ischemia reperfusion tissue,reducing neu- trophil adhesion and infiltration together with the inflammatory exudate effectively,inhibit the permeability of microcirculation,reduce the muscle injury of lower limb.