应用Sos恢复系统筛选与番木瓜环斑病毒CI互作的寄主因子

Screening of Proteins Interacting with PRSV CI in Carica papaya

应用Sos恢复系统(Sos recruitment system)筛选可能与番木瓜环斑病毒CI(Cylindrical inclusion protein,圆柱形内含体蛋白)相互作用的寄主蛋白因子,通过营养缺陷、温度敏感筛选、回转验证、序列测定和同源比对分析,获得1种候选的互作寄主蛋白,该蛋白与植物的叶绿体延伸因子Tu(Elongation factor Tu,EF-Tu)具有较高的相似性,并对其在番木瓜环斑病毒(Papaya Ringspot Virus,PRSV)致病与植物防御过程中的功能进行初步分析。

The cylindrical inclusion（CI） protein of Papaya ringspot virus（PRSV）is the key protein which is invovlved in virus replication and cell-to-cell movement.Using PRSV CI as the bait,one protein from a papaya plants cDNA library was identified by a Sos-Recruitment system.Sequence alignment analysis revealed that this host protein was highly homology to Elongation factor Tu（EF-Tu）from different plant species.The possible roles of this host protein in PRSV infection and host defense were discussed,which provided some new clues in elucidating the molecular mechanisms of PRSV infection and host defense.