摘要
目的筛选毛脉酸模叶有效成分的最佳提取工艺。方法以毛脉酸模叶中白藜芦醇苷、白藜芦醇、大黄素及出膏率为考察指标,用正交设计试验优化工艺,并通过多指标综合评分比较3种方法。结果传统索氏提取法最佳提取工艺的综合评分为35.59;复合酶法最佳提取工艺的综合评分为86.41;通过提取原生质体得到有效成分的方法的最佳提取工艺的综合评分为92.17。结论通过提取叶片中原生质体得到有效成分的方法要优于另外两种方法。
Objective To compare the diffirent extracting methods on effective components in leaf of Rumex gmelini.Methods Use resveratrol glucoside,resveratrol,emodin and paste-forming rate as the indictator,adopt orthogonal design testing and multiple guidelines grading method to compare three methods.Results The grade of the best extraction technology of the traditional soxhlet extraction method was 35.59;compound enzyme method was 86.41;the method of extracting protoplast was 92.17.Conclusion The method of extracting protoplast is better than the other two methods.
出处
《中国药业》
CAS
2011年第9期4-7,共4页
China Pharmaceuticals
基金
国家自然科学基金项目
项目编号:30970300
30270156
关键词
毛脉酸模
提取方法
最佳提取工艺
Rumex gmelini Turcz.
extracting methods
best extraction technology