Smo和Gli1蛋白在食管鳞状细胞癌组织中的表达及临床意义

Clinicopathological significance of expression of Smo and Gli1 in esophageal squamous cell carcinoma

目的:探讨Sonichedgehog信号通路中Smo和Gli1蛋白在食管鳞状细胞癌(ESCC)组织中的表达及临床意义.方法:采用免疫组织化学二步法分别检测60例ESCC、45例癌旁异型增生及60例食管正常黏膜组织中Smo和Gli1蛋白的表达,并分析两者的表达水平与临床病理因素的关系.结果:Smo和Gli1蛋白在ESCC组织中表达强度显著高于癌旁异型增生和正常食管黏膜组织(53/60vs22/45,9/60;54/60vs18/45,16/60,均P<0.05),而Smo和Gli1蛋白在癌旁异型增生及食管正常黏膜组中的表达差异无统计学意义.Smo和Gli1蛋白在ESCC组织中的表达强度与肿瘤的浸润深度和淋巴结转移有关(均P<0.05).相关分析显示Smo和Gli1蛋白在ESCC组织中的表达呈正相关(r=0.452,P<0.01).结论:Sonic hedgehog信号通路异常激活与ESCC的发生发展密切相关,在食管鳞状细胞癌中Sonic hedgehog信号通路的激活可能是高表达的Smo蛋白通过上调其下游的转录因子Gli1蛋白来发挥作用的,联合检查两者对ESCC患者预后的判断有一定的临床意义.

AIM：To investigate the expression of Smo and Gli1 proteins in esophageal squamous cell carcinoma（ESCC）.METHODS：The protein expression of Smo and Gli1 was detected by immunohistochemistry in 60 ESCC specimens,45 tumor-adjacent atypical hyperplastic tissue specimens,and 60 normal esophageal mucosal specimens.RESULTS：The expression of Smo and Gli1 protein was significantly higher in ESCC than in atypical hyperplastic tissue and normal esophageal mucosal tissue（53/60 vs 22/45,19/60;54/60 vs 18/45,16/60;all P 0.05）.There was no signif icant difference in the expression of the two proteins between atypical hyperplasia tissue and normal esophageal mucosal tissue（both P 0.05）.Overexpression of Smo and Gli1 proteins was related with lymph node metastasis and the depth of tumor invasion in ESSC（both P 0.05）.The expression of Smo protein was signif icantly correlated with that of Gli1 protein in ESCC（r = 0.452,P 0.01）.CONCLUSION：Abnormal sonic hedgehog signaling may play an important role in the development and evolution of ESCC,and this abnormality is associated with Smo protein overexpression,which can upregulate the expression of Gli1 protein,a downstream transcription factor in the sonic hedgehog signal transduction pathway.Combined detection of Smo and Gli1 proteins may be helpful to evaluate the prognosis of ESCC.