孕激素在小鼠外阴阴道念珠菌病模型中的作用

Role of Progesterone in Mouse Model with Vulvovaginal Candidiasis

目的探讨孕激素在雌激素诱导小鼠外阴阴道念珠菌病模型中的作用。方法 8～10周龄雌性Balb/c小鼠在阴道接种10μL白念珠菌悬液(5×104个孢子)前72h皮下注射不同剂量雌二醇和/或黄体酮,接种后3,7,14和21d各组随机取2只小鼠进行阴道菌落计数和组织病理检查。结果各组菌落计数在接种后3d最高,7d明显减少,21d几乎为0。与E0P0组比较,单用雌激素组在3d和7d菌落计数均明显升高(P<0.05),但单用孕激素组无明显变化(P>0.05)。单用雌激素组的阴道黏膜炎症反应比单用孕激素组出现早、程度重。结论单用孕激素不能有效诱导小鼠阴道白念珠菌感染,但联用雌激素可起到促进或协同作用。

Objective To investigate the role of progesterone in estrogen-induced vulvovaginal candidiasis（VVC）of mice.Methods Female Balb/c mice aged 8 to 10 weeks were subcutaneously injected with different doses of estradiol and/or progesterone on day 3 before vaginal inoculation of 10μL Candida albicans suspension（5×10^4 cells）.Two mice in all groups were randomly selected for vaginal colony count and histopathologic examination on day 3,7,14 and 21 post-inoculation.Results The colony counts in all groups peaked on day 3,decreased remarkably on day 7,and reached a nadir on day 21 after inoculation.Compared with E0P〈0 group,the colony counts increased significantly in estrogen groups（P〈0.05）,but showed no change in progesterone groups（P〈0.05）.The inflammatory response of vaginal mucosa in estrogen groups occurred earlier and more heavily than that in progesterone groups.Conclusion Simple administration of progesterone is unsatisfactory for inducing vaginal Candida albicans infection,but combined treatment with estrogen and progesterone can achieve the promotive or synergistic effect.