金黄色葡萄球菌致小鼠慢性输卵管炎性不孕模型制作

Modeling Chronic Salpingitis Infertility with Staphylococcus aureus in Mice

目的通过金黄色葡萄球菌直接感染小鼠输卵管,建立炎症致不孕的动物模型。方法用1×109/mL的金黄色葡萄球菌接种小鼠,制作慢性输卵管炎症模型,观察输卵管病理炎性改变以及小鼠的受孕情况。结果造模术75 d后,模型组小鼠受孕率、输卵管通畅率显著低于对照组(P<0.001)。模型组肉眼观察输卵管有不同程度积水积脓、僵硬,输卵管与周围组织均有不同程度的粘连;病理学观察输卵管管腔被异物肉芽组织完全阻塞,全层均见大量慢性炎细胞浸润。结论输卵管内接种浓度1×109/mL的金黄色葡萄球菌可以成功建立小鼠输卵管炎性不孕模型。

Objective To establish infertile animal model through inflammation by directing infection mouse oviduct with staphylococcus aureus.Methods Directing inoculation concentration of 1 × 109 / mL of staphylococcus aureus into mice tube,making the model of chronic inflammation in the fallopian tube,observing the pathological inflammatory changes and pregnancy of mice.Results Seventy-five days later,mouse pregnancy rates and tubal patency rate in model group were significantly lower than control group（P 0.001）.In model group,the tubes had different degrees of dropsy,empyema and rigid tube had different levels of adhesion with surrounding tissue by visual observation.The fallopian tube lumen was completely obstructed by foreign granulation tissue and the whole layer was full of a large number of chronic inflammatory cell infiltrations by pathological observation.Conclusion That the concentration of 1 × 109 / mL of Staphylococcus aureus were inoculated could successfully establish a mouse model of salpingitis infertility.