摘要
目的比较鸡胚尿囊膜试验(HET-CAM)作为一种眼刺激替代方法对产品或原料的评价。方法采用HET-CAM和兔眼Draize试验方法,对19种原料和23种化妆品及家用洗涤产品眼刺激性进行检测,对体内体外试验的结果进行统计比较。结果比较体内体外试验,原料和产品的kappa系数分别为0.826,0.531;灵敏度分别为100%,81.8%;特异度分别为85.7%,77.8%。结论 HET-CAM可作为Draize试验的替代试验,HET-CAM系统更适合对单纯化学品原料进行眼刺激试验。
Objective To compare the adaptability of HET-CAM eye irritation test for products and ingredients.Methods Test 19 ingredients and 23 products by HET-CAM and Draize eye irritation protocol.Compare the data from in vitro and in vivo test.Result Compare in vitro data and in vivo data,The Kappa coefficient,sensitivity,specificity are 0.826,100%,85.75 for ingredient.0.531,81.8%,77.8% for cosmetics as well.Conclusion HET-CAM eye irritation test can be used to be alternative test of Draize test.HET-CAM system is more suitable for ingredients test.
出处
《中国比较医学杂志》
CAS
2011年第3期27-30,35,81,共6页
Chinese Journal of Comparative Medicine
