精子冷冻保存技术及研究进展

Progress on Sperm Cryopreservation

精子冷冻是辅助生殖技术的基础,能够有效的保存有价值的基因资源。文章回顾了近些年来国内外精子冷冻保存的重要研究成果,分析了精子的来源、冷冻预处理、冷冻和解冻方法、防冻剂的选择及其加入去除方式的选择等因素对精子冷冻效果的影响。文章还总结了精子冷冻效果的评价方法,展望精子冷冻技术的发展方向和应用前景。

Sperm cryopreservation has revolutionized the field of assisted reproduction,and it can provide an effective way to preserve valuable genetic resources.This review contained information relating to the cryopreservation of animal semen through a summary of significance research results about domestic and foreign sperm cryopreservation in recent years,and the topics discussed that the effect of semen collection,freezing pretreatment,freezing and thawing methodologies,cryoprotectant selection and methods of its addition and removal on sperm cryopreservation.In addition,this review also focus on evaluation criteria for post-thaw spermatozoa,proposing prospect on anticipation about research orientation of sperm cryopreservation and its application.