中国麻风树种质资源SSR遗传多样性的研究初报

SSR-based Genetic Diversity of Jatropha curcas Germplasm in China

麻风树(Jatropha curcas)是世界上最有潜力的能源植物之一。本研究针对来自全国6省区的219份麻风树种质资源,应用SSR分子标记对其进行了初步的遗传多样性分析。根据公共数据库中的所有序列设计出了20对SSR及4对eSSR引物,这些引物均能很好地扩增麻风树DNA片段。在上述24个SSR标记中,有8个(33.33%)探测到麻风树群体的DNA多态性。这24个SSR标记共扩增出36个位点,平均每个标记扩增1.5个位点,其中12个(33.33%)位点显现出多态性。这表明中国麻风树是一个具有中等SSR多态性水平的物种,其多态百分率为33.3%。

Jatropha curcas is an oil-bearing plant with multiple uses and great potential for ener-gy application.In the present study,we preliminarily investigated genetic diversity of Chinese J.curcas germplasm by using SSR markers.A set of 219 J.curcas samples from all adaptation areas in China was adopted.Twenty pairs of SSR primers and four pairs of eSSR primers were designed based on the public database for nucleotide sequences of J.curcas.These SSR or eSSR well amplified all DNA samples.Among the 24 SSR or eSSR markers,eight（33.3%）detected polymorphism in the J.curcas population.The 24 SSR or eSSR markers amplified 36 loci in total,of which 12（33.33%） showed polymorphism.The average number of loci amplified by the SSR markers was 1.5.Therefore,there is a modest level of genetic diversity in Chinese J.curcas germplasm.