甘蓝型油菜(EruCMS)与甘蓝种间杂种的同工酶和蛋白质分析

Preliminary Studies on Isozymes and Protein of Hybrid from Brassica napus×Brassica oleracea

经胚抢救获得甘蓝型油菜(Brassica napus L.)(EruCMS)与甘蓝(Brassica oleracea L.var.capitata L.)种间杂种,前期经过流式细胞仪、柱头染色体数目、花粉活力等分析获得一些真杂种。利用电泳法,对真杂种植株的3种同工酶(SOD、EST、COD)和蛋白质进行详细分析,了解了杂种与亲本的同工酶和蛋白质的特性差异。结果表明,杂种与亲本之间的同工酶和蛋白质存在较明显的差异:杂种的SOD、COD的酶带表现为偏父本甘蓝型;杂种的EST的酶带表现为偏母本油菜型;杂种的蛋白质电泳表现为不仅具有双亲的特征蛋白带,也有其自身特征蛋白带。

Brassica napus×Brassica oleracea hybrids were obtained by embryo rescue tech-nology and identified by flow cytometry analysis,chromosome count of stigma cells,and pollen viability tests.Patterns of isozyme and protein of the real hybrids were studied by polyacrylamide gel electrophoresis.In addition,isozyme（SOD,EST,and COD） and protein patterns were compared with those of the parents,with obvious variations observed.Results also showed that the SOD and COD patterns of the hybrids were similar to Brassica oleracea,while the EST pattern was similar to the female parent.The protein pattern mainly showed parental complements,with new bonds also possessing their own characters.