土炒对白术中白术内酯Ⅰ、Ⅱ、Ⅲ含量的影响

Contents Comparision of AtractylodeⅠ、Ⅱ、Ⅲ in Rhizoma Atractylodis Macrocephalae and the Processed with Soils

目的:探讨土炒对白术有效成分白术内酯Ⅰ、Ⅱ、Ⅲ含量的影响,并通过与清炒白术的比较,研究辅料土对白术成分变化是否具有实质作用。方法:采用高效液相色谱法测定各样品中白术内酯Ⅰ、Ⅱ、Ⅲ的含量。采用ODS C18柱(200 mm×4.6 mm,5μm),流动相:乙腈-水梯度洗脱,流速:1.0 mL/min,柱温:30℃,检测波长:白术内酯Ⅰ、Ⅲ为220 nm;白术内酯Ⅱ为276 nm。结果:白术内酯Ⅰ、Ⅱ、Ⅲ测定方法学考察符合液相色谱法的要求;生白术中白术内酯Ⅰ、Ⅱ、Ⅲ含量分别为0.4365、0.2878、0.4140 mg/g,10批土炒白术中白术内酯Ⅰ、Ⅱ、Ⅲ平均含量分别为0.5503、0.3013、0.8403 mg/g,10批清炒白术中白术内酯Ⅰ、Ⅱ、Ⅲ平均含量分别为0.5386、0.2958、0.7399 mg/g。结论:白术经炮制后白术内酯Ⅱ含量不变,白术内酯Ⅰ、Ⅲ显著升高,清炒和土炒对白术内酯类成分含量影响无显著差异。

Objective：To research the effect of processing with soils on the contents of Atractylode Ⅰ、Ⅱ、Ⅲ of Rhizoma Atractylodis Macrocephalae.Methods：Contents of Atractylode Ⅰ、Ⅱ、Ⅲ were determined by HPLC.Hypersil-ODS C18（200 mm×4.6 mm,5 μm）column was used;The mobile phase consisted of acetonitrile-water;The flow rate was 1.0 mL/min;The detection wavelength was 220 nm and 276 nm,the column temperature was 30 ℃.Results：All the indexes of Atractylode Ⅰ、Ⅱ、Ⅲ peak were qualified;The result of methodological study was met the requirement of HPLC;The average contents of Atractylode Ⅰ、Ⅱ、Ⅲ in raw Rhizoma Atractylodis Macrocephalae were 0.4365、0.2878、0.4140 mg/g,the soil-processed Rhizoma Atractylodis Macrocephalae were 0.5503、0.3013、0.8403 mg/g,the processed Rhizoma Atractylodis Macrocephalae were 0.5386、0.2958、0.7399 mg/g.Conclusion：The content of Atractylode Ⅱ is no significant difference among the raw Rhizoma Atractylodis Macrocephalae and the Processed.The contents of both Atractylode Ⅰand Ⅲ increased after processed.However,there is no significant difference between the soil-processed and the processed Rhizoma Atractylodis Macrocephalae with nothing.