通城移植毛喉鞘蕊花化学成分及抗肿瘤活性研究

Studies on the Chemical Constituents of Coleus forskohlii Transplanted in Tongcheng and Their Antitumor Activity

目的:研究通城移植毛喉鞘蕊花全草的化学成分。方法:利用硅胶柱色谱、制备薄层色谱和ToyopearlHW-40F凝胶渗透进行分离、纯化。根据理化性质和现代波谱技术并辅以化学方法进行结构鉴定。结果:从通城移植毛喉鞘蕊花中分离得到7个化合物,分别鉴定为:羽扇豆醇(1)、齐墩果酸(2)、熊果醇(3)、β-谷甾醇(4)、colonicacid(5)、去甲基柳杉树脂酚(6)、coleolic acid(7)。抗肿瘤实验结果表明化合物1、2、6、7均具有抗肿瘤活性。结论:化合物1、3为首次从该属植物中得到;化合物1为首次从该植物中发现的抗肿瘤活性成分。

Objective：To study the chemical constituents of Coleus forskohlii.Methods：Isolation and purification were carried out by silica gel column chromatographic and Toyopearl HW-40F.Compounds were identified and elucidated by spectral and chemical methods.Results：Seven compounds were obtained from ethyl acetate extract fraction.Their structures were identified as lupeol（1）,oleanolic acid（2）,uvalo（3）,β-sitosterol（4）,colonic acid（5）,demethylcryptojaponol（6）,coleolic acid（7）.Compounds 1,2,6,7 showed obviously antitumor activity.Conclusion：Compound 1 and 3 are isolated from the genus for the first time.Moreover,compound 1 is firstly found to have antitumor activity from the plants.