小檗碱对高脂-高盐-果糖大鼠肾损伤的影响及其机制

Effects and Mechanism of Berberine on the Hypertensive Renal Injury Rats Induced by Enriched High Fat-salt-fructose Diet

目的:探讨小檗碱对高脂-高盐-果糖诱致大鼠肾损伤保护作用的抗氧化机制。方法:取SD大鼠50只,随机分为正常对照组和造模组。造模组大鼠给予高脂-高盐饲料喂养,并交替饮用糖盐水(糖4%、盐1%)和果糖水(6%)8 w。根据血压情况,随机将高血压的动物分为模型组(10只,蒸馏水)、卡托普利(10只,25 mg/kg)组、小檗碱高剂量组(10只,300 mg/kg)和小檗碱低剂量组(10只,100 mg/kg)。继续给予高脂-高盐-果糖饲养,同时分别灌胃给予上述药物处理4 w。分别在0、4、8、10、12周末测定血压,药物处理4 w后,留取尿液测定尿蛋白含量,取血测定血肌酐和血尿素氮,分离血清测定SOD活性和MDA、GHb含量,测定肾脏组织H2O2、GSH-Px含量和CAT活性。结果:模型大鼠血压、尿蛋白、血肌酐和血尿素氮水平显著高于正常大鼠(P<0.01),并伴有血清SOD活性降低、MDA和GHb含量升高(P<0.01),肾脏组织H2O2含量升高,降低GSH-Px含量和CAT活性。小檗碱处理4 w,显著降低模型大鼠的血压、尿蛋白、血肌酐和血尿素氮水平(P<0.01),提高SOD活性,降低MDA和GHb含量(P<0.01),同时提高肾脏组织GSH-Px和CAT活性(P<0.01),轻度降低肾脏组织H2O2含量。结论:小檗碱对高脂-高盐-果糖诱致肾损伤模型大鼠具有保护作用,其作用机制可能与提高机体和肾脏组织的抗氧化能力有关。

Objective：To study the effects of berberine on hypertensive renal injury model in rats fed by enriched high fat-salt-fructose diet.Methods：hypertensive renal injury model was esteblished by feeding enriched high fat-salt-fructose diet for 8 weeks.On the basis of animal blood pressure,hypertensive rats were randomly divided into model group（10 rats,distilled water）,captopril group（10 rats,25 mg/kg）,berberine high dose group（10 rats,300 mg/kg） and low dose group（10 rats,100 mg/kg）.These rats were fed by enriched high fat-salt-fructose diet and treated by intragastric administration with drugs for 4 weeks.And normal control group（10 rats）was set Blood pressure was determined at 0,4,8,10,12 weekend,and after 4 weeks of drugs treatment,getting urine to determine urine protein,taking blood serum to determine blood urea nitrogen,serum creatinine,GHb,MDA and activity of SOD.The content of H2O2 and GSH-Px and activity of CAT in kidney tissues were determined also.Results：Compared with normal control group,blood pressure,urine protein,blood urea nitrogen,serum creatinine and MDA and GHb in serum of model rats obviously increased （P0.01）,the activity of SOD decreased（P0.01）,higher content of H2O2 and lower content of GSH-Px and activity of CAT（P0.01） in the kidney tissues.Treated with berberine for 4 weeks,elevated blood pressure and heightened levels of urine protein,blood urea nitrogen and serum creatinine in model rats were depressed significantly（P0.01）,and elevated the activity of SOD,lowed the levels of MDA and GHb in blood serum（P0.01）.At the same time,berberine increased the activities of GSH-Px and CAT（P0.01） and slightly lowed the content of H2O2 in the kidney tissues.Conclusion：Berberine has protecting effects on the hypertensive renal impairment model rats fed by enriched high fat-salt-fructose diet,which are concerned with elevated antioxidant capability in body and kidney tissues.