镰形棘豆总黄酮霜防紫外线损伤作用研究

Study on the Ultraviolet Protective Effects of the Cream of the Total Flavonoids from Oxytropis falcata

目的:研究镰形棘豆总黄酮霜对中波紫外线(280～320 nm,UVB)致大鼠皮肤损伤的防护作用。方法:采用涂霜(10 mg/cm2)后再照射5 min的方法,连续照射1 w;对各组大鼠皮肤组织进行外观和显微学观察;用比色法测定大鼠皮肤组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、羟脯氨酸(Hyp)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)、谷胱甘肽-S转移酶(GST)、过氧化氢酶(CAT)和羟自由基(.OH)的活力或含量。结果:镰形棘豆总黄酮霜能有效防止紫外线照射对皮肤造成的外观和病理学损伤,提高皮肤组织中SOD(P<0.001)、GSH-Px(P<0.001)、GST(P<0.05)、CAT(P<0.01)活性及Hyp含量(P<0.001),降低MDA、.OH的含量(P<0.001)及GSH的活性(P<0.001)。结论:镰形棘豆总黄酮霜能有效防护紫外线,防止皮肤损伤。

Objective：To study the protective effects of the cream of the total flavonoids from Oxytropis falcata on the destructed skin of mice induced by moderate-wave ultraviolet（UVB） irradiation.Methods：Dorsal skin of Wistar mice were treated with the cream of the total flavonoids from Oxytropis falcata and then irradiated with UVB in the dosage of 5 min once a day for one week.The tissue of skin was pathological diagnosed and the activities or contents of superoxide dismutase（SOD）,malondialdehyde（MDA）,hydroxyproline（Hyp）,glutathione peroxidease（GSH-Px）,glutathione（GSH）,glutathion-s-transferase（GST）,catalase（CAT） and hydroxy radical（·OH） were determined with chromatometry.Results：The ultraviolet protective effects of the cream could be observed with appearance and pathology examine.The cream could increase the activities of SOD（P0.001）,GSH-Px（P0.001）,GST（P0.05） and CAT（P0.01）,raise the content of Hyp（P0.001） significantly.The cream could also decrease the contents of MDA and ·OH（P0.001）,and the activities of GSH significantly（P0.001）.Conclusion：The cream of the total flavonoids from Oxytropis falcata has protective effect on the destructed skin of mice induced by moderate-wave ultraviolet（UVB） irradiation.