瓜馥木总黄酮提取纯化及其清除自由基活性研究

Study on Extraction and Purification Techniques and Free Radical Scavenging Activity of Total Flavonoids from Fissistigma oldhamii

目的:研究瓜馥木总黄酮最佳提取工艺,并将总黄酮进行富集纯化,测试其清除自由基活性。方法:采用正交实验,考察乙醇浓度、提取温度、固液比及提取时间4个因素对该工艺的影响;利用AB-8大孔吸附树脂分离纯化总黄酮粗品;采用清除DPPH自由基初步评价瓜馥木提取物及总黄酮的抗氧化能力。结果:确立了瓜馥木总黄酮的最佳提取工艺为:提取时间3 h,乙醇浓度90%,固液比1∶10,提取温度为70℃。黄酮粗品经AB-8大孔树脂纯化以后,含量可达到55%以上。结论:优选出的最佳提取工艺条件及纯化总黄酮的方法对进一步的工艺开发有较好的参考价值,瓜馥木富集纯化的总黄酮部位具有较强的清除自由基能力,为开发成为中药保健品提供了一定的依据。

Objective：To optimize the extraction and purification techniques of total flavonoids in Fissistigma oldhamii.The scavenging activity to DPPH of the total flavonoids and the extraction were studied in vitro.Methods：By orthogonal design,we investigated the effects of concentration of ethanol,temperature,ratio of solid to liquid and time of extraction on the total flavonoids extraction rate.The total flavonoids was separated and purified by AB-8 macroporous resin absorption.Results：The optimum extraction condition was that at extraction time of 3 h,concentration of alcohol 90 %,solid/liquid ratio of 1∶10 and temperature of 70 ℃.The content of total flavonoids in the refined product was above 55%.The GFM-2 fraction showed antioxidant capacity in vitro.Conclusion：The optimum extraction condition and purification experiment are very useful for further development of industrial process.Meantime,the result may be helpful for further development of Fissistigma oldhamii as health protection foods.