不同分离提纯工艺对冬凌草多糖提取率与免疫活性的影响

Effect of Separation and Purification Processes on Extraction Ratio of Polysaccharides in Rabdosia rubescens and Immunocompetence

目的:优选分离冬凌草多糖的方法,探究不同分离方法对冬凌草多糖分离效果的影响。方法:分别以DEAE-Sepharose Fast Flow(Cl-型)弱极性阴离子交换层析法、乙醇分级醇析法对冬凌草粗多糖进行分离纯化,以不同分离组分中多糖含量及其免疫活性比较优选冬凌草粗多糖的分离工艺。结果:DEAE-Sepharose Fast Flow(Cl-型)弱极性阴离子交换层析法分离得到5个组分,通过乙醇分级醇沉初步得到4个冬凌草多糖组分,前者分离产品纯度较高,而后者产品在体外小鼠脾淋巴T细胞的增殖实验中表现出较强生物活性。结论:为进一步研究冬凌草多糖的生物活性,可选用乙醇分级醇沉法对冬凌草多糖进行分离纯化。

Objective：To select a good method for purifying polysaccharides from Rabdosia rubescens,and study their immunological activity.Methods：DEAE-Sepharose Fast Flow（Cl-）weak polar anion-exchange chromatography and stepwise ethanol precipitation were used to isolate and purify the polysaccharides from Rabdosia rubescens,respectively.MTT method was used to evaluate the immune activity.Results：Five components were obtained from DEAE-Sepharose Fast Flow（Cl-）weak polar anion-exchange chromatography and four polysaccharides were obtained through alcohol precipitation.Conclusion：Polysaccharides from Rabdosia rubescens by stepwise ethanol precipitation show stronger immunological activity.Their immunomodulatory effect and related mechanism will be researched further.