苯酚对厌氧颗粒污泥的毒性研究

Study on Toxicity of Phenol to Anaerobic Granular Sludge

通过改变苯酚的浓度,研究厌氧颗粒污泥的产甲烷比活性(SMA)、胞外多聚物(EPS)产生量和组分多糖、蛋白质以及辅酶F420的变化情况,以明确苯酚对厌氧颗粒污泥的毒性作用影响,并采用红外光谱分析了不同浓度苯酚作用对EPS分子结构的影响.结果表明,苯酚浓度<100 mg/L时,对厌氧颗粒污泥的影响很小,污泥的产甲烷活性、辅酶F420和EPS都基本没发生变化.当苯酚浓度从100 mg/L上升至400 mg/L时,厌氧颗粒污泥的产甲烷比活性从173.4 mL/(g.d)下降至57.6 mL/(g.d)[对照组为259.6 mL/(g.d)];厌氧颗粒污泥VSS中辅酶F420含量从0.14μmol/g减少至0.08μmol/g(对照组为0.154μmol/g);厌氧颗粒污泥VSS中EPS的总量(用TOC表示)从33.1 mg/g降低至15.0 mg/g(对照组为38.5 mg/g).当苯酚浓度增加至800 mg/L时,从红外光谱分析EPS分子结构表明,EPS的分子结构发生了改变,苯酚对EPS分子结构影响很大.

To understand the toxicity of phenol to anaerobic granular sludge,the changes of specific methanogenic activity（SMA）,extracellular polymeric substance（EPS） constituents and coenzyme F420 concentration of anaerobic granular sludge at various phenol concentrations were investigated.Meanwhile,Fourier transform infrared spectrometry was used to analyze the structure of EPS.The results show that the phenol has little effect on anaerobic granular sludge when the concentration of phenol is less than 100 mg/L.When the phenol concentration increases from 100 mg/L to 400 mg/L,the special methanogenic activity（SMA） decreases from 173.4 mL/（g.d） to 57.6 mL/（g.d）,coenzyme F420 concentration decreases from 0.14 μmol/g to 0.08 μmol/g,and EPS concentration,which is represented by TOC,decreases from 33.1 mg/g to 15.0 mg/g,when the SMA and the concentration of coenzyme F420 and EPS in the blank test are 259.6 mL/（g.d）,0.154 μmol/g and 38.5 mg/g respectively.The analysis of molecular structure of EPS with Fourier transform infrared spectrometry shows that it has big effect on molecular structure of EPS when the phenol concentration is 800 mg/L.