应用聚合酶链式反应检测禽痘病毒田间分离株及疫苗株中网状内皮组织增殖病毒LTR片断,env及re1基因

Detection of Reticulonendotheliosis Virus(REV)LTR Fragments env and rel Genes in Fowlpox Virus Vaccine and Field Strains by Using Polymerase Chain Reaction

本文利用聚合酶链式反应对来自澳大利亚昆士兰州、维多利亚州和新南维尔士州的４株禽痘病毒田间分离株及二疫苗株中网状内皮组织增殖病毒的ＬＴＲ片断、ｅｎｖ及ｒｅｌ基因因进行了检测。所有４株痘病毒分离株均为ＬＴＲ片断、ｅｎｖ阳性，曾被ＰＣＲ证实为ＬＴＲ阳性的一疫苗株在本实验中也为ＬＴＲ、ｅｎｖ阳性，另一疫苗株为ＬＴＲ、ｅｎｖ阴性，所有上述禽痘病毒株均为ｒｅｌ阴性。

Summary.Polymerase chain reaction(PCR)was used to detect RE long terminal Repeat(LTR)fragments,env and rel genes in 4 fowlpox virus(FPV)field isolates FPV19,FPV59,FPV60,FPV62 in Queensland(QLD),Victoria(VIC) and NEW South Wales(NSW) and 2 FPV vaccine strains FPV30 and FPV31 which were Webster's FPV standard and mild vaccine strains.All 4 field strains and FPV30 were REV LTR and env positive while FPV31 was REV LTR and env negative.All 6 FPV vaccine and field strains were rel negative.