荧光碳纳米粒子的制备及其在细胞标记中的应用

Preparation of fluorescent carbon nanoparticles and application in the cell labeling

在生物医学领域,对细胞、生物分子进行染色、修饰标记和检测一直是生物医学分析的重要内容。近年来,纳米材料与生物检测技术的结合,使得生物分子的检测有了重要的发展。用30%H2O2/ACOH(V∶V=2∶1)混合氧化性酸溶液回流蜡烛烟炱制备得到荧光碳纳米粒子,用不同浓度的荧光碳纳米粒子对Hela细胞进行荧光标记,在激光共聚焦荧光显微镜下观察得到荧光标记图像。结果表明,荧光碳纳米粒子能够有效地对Hela细胞进行荧光标记。荧光碳纳米粒子具有无毒、化学惰性和良好的生物相容性,可进行多色标记,并且发光强度高,光化学稳定性好,在生物医药领域具有重要的应用价值。

In biological and medical fields,one of the important content in biomedical analysis is to stain,modify,labell and detect cells and biomolecules.In recent years,combining nano-materials and bio-detection technology makes an important development of the detection of biological molecules.In this work multicolor fluorescent carbon nanoparticles（CNPS） were prepared from the combustion soot of candles by refluxing in a mixed oxidants（30%H2O2/ACOH in V∶V=2∶1）.Hela cells were labeled with varied concentrations of solutions of fluorescent CNPs and observed by the confocal laser scanning fluorescence microscope.Experimental results have shown that the fluorescent CNPS are excellent biomarkers for Hela cells.Owing to their advantages such as non-toxic,chemical inertness,biocompatibility,multi-colored marking,high luminous intensity and photochemical stability,fluorescent CNPs are hopefully qualified to be biomarker-candidates which are important in the application in biological and medical fields.