肝外胆管癌中Ki-67表达及微血管密度与临床病理特征的关系

Relationship between expression of Ki-67,microvessel density and pathological characteristics in extrahepatic cholangiocarcinoma

[目的]探讨肝外胆管癌中Ki-67表达和微血管密度与病理特征的关系.[方法]应用免疫组织化学SP法观察40例肝外胆管癌组织及10例癌旁黏膜组织标本中Ki-67表达,用CD 34抗体标记血管内皮细胞,并检测Ki-67标记指数和微血管密度,分析其与肝外胆管癌病理特征的相关性.[结果]肝外胆管癌组织中Ki-67标记指数为37.2%±5.1%,癌旁黏膜组织为8.9%±1.4%,差异具有统计学意义(P<0.05);肝外胆管癌中微血管密度值为56.5%±9.0%,癌旁黏膜组织中为20.6%±3.3%,差异亦具有统计学意义(P<0.05);Ki-67的标记指数和微血管密度与肝外胆管癌的分化程度、有无淋巴结转移及TNM分期均有明显相关性(P<0.05).肝外胆管癌组织中Ki-67标记指数与微血管密度呈正相关.[结论]Ki-67标记指数和肿瘤微血管密度与肝外胆管癌的病理特征密切相关,可作为评价肝外胆管癌生物学特性的指标.

OBJECTIVE To study the relationship between expression of Ki-67,microvessel density（MVD） and pathological characteristics in extrahepatic cholangiocarcinoma（EHCC）.METHODSExpression of Ki-67 and CD34 was detected by immunohistochemical SP method in 40 cases of EHCC and 10 cases of mucosa adjacent to EHCC.The labeling index（LI） of Ki-67 and MVD values were measured,and its correlation with the pathological characteristics of EHCC was analyzed.RESULTSThe LI of Ki-67 were 37.2%±5.1% and 8.9%±1.4%,and MVD values were 56.5%±9.0% and 20.6%±3.3%（P0.05） in EHCC and mucosa adjacent to EHCC,respectively.The LI of Ki-67 and MVD were related with differential degree,lymphnode metastasis and TNM stages of EHCC,and there was positive correlation between the LI of Ki-67 and MVD value.CONCLUSIONThe LI of Ki-67 and MVD are closely related with pathological characteristics in EHCC and the Ki-67 and MVD can be provide to target for judging biological characteristics of EHCC.