CMV感染新生儿尿DNA含量和血T细胞亚群变化

CHANGES OF DNA CONTENT IN URINE AND T CELL SUBSETS IN BLOOD OF NEONATES WITH CYTOMEGALOVIRUS INFECTION

目的探讨巨细胞病毒（CMV）感染新生儿尿DNA含量和血T细胞亚群的变化及意义。方法选择CMV症状性感染新生儿20例,CMV无症状性感染新生儿23例,健康查体新生儿16例,检测各组尿DNA含量和血T细胞亚群的水平,并对CMV感染病儿进行随访。结果 CMV症状性感染组尿DNA含量明显高于CMV无症状性感染组,差异有显著性（t=3.698,P〈0.01）。CMV症状性感染组CD3＋、CD4＋的水平和CD4＋/CD8＋的比值较无症状性感染组和健康对照组均降低,CD8＋水平较其他组升高,差异有显著性（F=3.74~42.34,q=3.21~11.76,P〈0.05、0.01）。随访显示CMV症状性感染组病儿中6例T细胞亚群的检测结果持续异常,并且肝功能反复,其中4例病人尿CMV DNA含量升高并维持在较高水平。结论 CMV症状性感染组尿DNA含量和血T细胞亚群较无症状性感染组有明显变化,此在判断预后和指导临床治疗方面有一定意义。

Objective To investigate the changes and significance of DNA content in urine and T cell subsets in blood of neonates infected with cytomegalovirus（CMV）.Methods Forty-three neonates who were confirmed infected by CMV were selected in this study： 20 cases were symptomatic,and 23 asymptomatic.Sixteen newborns who received health examination served as controls.The DNA content in urine and T cell subsets in blood were detected.The sick neonates were followed up.Results The urine DNA level in symptomatic sick neonates was higher than that in the asymptomatic,the difference being significant（t=3.698,P0.01）.Blood levels of CD3＋,CD4＋ and CD4＋/CD8＋ in the symptomatic group were lower than that in the asymptomatic and healthy controls,while the levels of CD8＋ were higher（F=3.74-42.34;q=3.21-11.76;P0.05,0.01）.Follow-up revealed that of the symptomatic sick neonates,six of them showed persistent T cell subsets,accompanying abnormal liver functions,of whom,urine DNA elevated in four cases and which was kept at a higher level.Conclusion Marked changes of urine DNA and blood T cell subsets were observed in symptomatic CMV-infected neonates,which is of significance in prediction of prognosis and in guidance of clinical therapy for this condition.