摘要
目的研究正常尿路上皮细胞的蛋白质表达谱,为尿路上皮癌蛋白质芯片的研制奠定基础。方法采用激光捕获显微切割技术从正常膀胱黏膜获得纯化的尿路上皮细胞,二维液相色谱电喷雾串联质谱鉴定标本中的蛋白质表达。应用生物信息学软件以及基因本体论(GO)工具分析鉴定蛋白质。结果共鉴定218个蛋白质,其中19个为假定蛋白质,11个为疏水性蛋白质,40个蛋白质等电点(PI)>9,42个蛋白质相对分子质量<104或者>105,13个蛋白具有跨膜结构。按GO划分218个蛋白质中具有生物学途径、细胞成分、分子功能注解的蛋白质分别为155、148、173个,浓集与缺失表达的GO术语分别为34/15、24/7、26/14个。结论本研究为尿路上皮癌生物标记组研究提供了有效的候选蛋白。
Objective To study the proteomic expression profile of normal human urothelium so as to establish basis for constructing urothelium carcinoma protein chips. Methods Pure urothelium cells from normal bladder epithelium were harvested by laser capture microdissection(LCM),and the proteomic expression profile was identified by two-dimensional liquid chromatography tandem mass spectrometry(2D-LC-MS/MS).The identified proteins were analyzed by bioinformatic softwares and gene ontology(GO) tools. Results A total of 218 proteins were identified,of which,19 were hypothetic proteins and 11 were hydrophobic proteins.There were 40(18.3%) proteins with PI 9 and 42(19.3%) proteins with MW104 or MW105,13 proteins had transmembrane structure.According to GO category,of the 218 proteins,155(71.1%) had biological process annotation,148(67.9%) with cellular component annotation and 173(79.4%) with molecular function annotation.The category enrichment/depletion in three ontologies were 34/15,24/7 and 26/14,respectively. Conclusion The results of this study present candidate proteins for the research of biomarker of urothelial carcinoma.
出处
《青岛大学医学院学报》
CAS
2011年第2期156-157,160,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
膀胱
上皮细胞
质谱分析法
urinary bladder
epithelial cells
mass spectrometry