eNOS基因多态性与老年射血分数保留心力衰竭相关性

RELATIONSHIP BETWEEN ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE POLYMORPHISM AND HEART FAILURE WITH PRESERVED EJECTION FRACTION IN ELDERLY PATIENTS

目的探讨内皮型一氧化氮合酶(eNOS)基因多态性与老年射血分数保留心力衰竭(HFPEF)的相关性。方法应用聚合酶链反应(PCR)、基因芯片技术等方法,检测230例老年HFPEF病人及200例健康者(对照组)基因型及基因频率,应用Logistic回归分析年龄、体质量指数(BMI)、血脂、血糖及基因型对老年HFPEF的影响。结果 HFPEF组eNOS基因G894T中GT+TT基因型频率及T等位基因频率与对照组比较差异均有显著性(χ2=8.341、3.654,P<0.05);Logistic回归分析显示,年龄、BMI、糖尿病、高血压、血总胆固醇、eNOS基因型是HFPEF发病的危险因素,OR值分别为3.252、1.223、1.434、1.845、4.008、1.378。结论年龄、BMI、糖尿病、高血压、血总胆固醇是HFPEF的危险因素;eNOS基因G894T可能是HFPPEF发病的易感基因。

Objective To investigate the relationship between endothelial nitric oxide synthase gene G894T polymorphism and heart failure with preserved ejection fraction（HFPEF） in elderly patients. Methods Polymerase chain reaction（PCR） and gene chip techniques were employed to study the genotype and allele frequencies in 230 patients with HFPEF and 200 healthy controls.Logistic regression was used to analyze the effects of age,BMI,serum lipid,blood sugar and genotype onHFPEF. Results The GT＋TT genotype frequency and T allele frequency of eNOS gene G894T were significantly different between the HFPEF group and the controls（χ2=8.341,3.654;P0.05）.Logistic regression study showed that age,BMI,DM,hypertension,TC and eNOS genotype were risk factors for HFPEF,the OR values were 3.252,1.223,1.434,1.845,4.008,and1.378,respectively. Conclusion This study suggests that age,BMI,DM,hypertension,and TC were risk factors for HFPEF,and eNOS gene might be a predisposing gene for HFPEF.