前列腺癌组织BMP2表达及其与DIF-1的相关性

EXPRESSION OF BONE MORPHOGENETIC PROTEIN 2 AND ITS ASSOCIATION WITH DIFFERENTIATION INHIBITING FACTOR 1 IN PROSTATE CANCER

目的探讨骨形态发生蛋白2(BMP2)在前列腺癌(PCa)及前列腺增生(BPH)组织中的表达及其与细胞分化的关系,并进一步分析其与分化抑制因子1(DIF-1)的相关性。方法采用免疫组化SP法,分别检测PCa和BPH组织中BMP2和DIF-1的表达,采用图像分析软件分析阳性表达的灰度和面积,并采用直线相关方法分析BMP2和DIF-1的相关性。结果 BPH组织中BMP2和DIF-1呈阴性或弱阳性表达;PCa组织中BMP2和DIF-1均呈阳性表达。在PCa组织中,BMP2和DIF-1的表达水平与Gleason评分呈正相关(rs=0.61、0.63,P<0.01);BMP2与DIF-1的表达也呈正相关(r=0.92,P<0.01)。结论在PCa组织中BMP2与DIF-1的表达存在相关性,且二者的表达均与PCa的恶性程度密切相关,提示二者可能与PCa的发生发展有关。

Objective To investigate the expression of bone morphogenetic protein 2（BMP2） in prostate cancer（PCa） and benign prostatic hyperplasia（BPH）,and its association with cell differentiation,and differentiation inhibiting factor 1（DIF-1）. Methods Expressions of BMP2 and DIF-1 PCa and BPH were detected using immunohistochemical streptavidin peroxidase（SP） method.The positive expressions were analyzed using image analysis software.Linear correlation analysis was applied to analyze the correlation of BMP2 with DIF-1. Results In BPH,weak or negative expression of both BMP2 and DIF-1 was observed;In PCa,both BMP2 and DIF-1 showed positive expression.In PCa,the expressions of BMP2 and DIF-1 were positively correlated with Gleason score（rs=0.61,0.63;P0.01）,and BMP2 was positively correlated with DIF-1（r=0.92,P0.01）. Conclusion In prostate cancer,there is a correlation between the expressions of BMP2 and DIF-1,their expressions are closely related with the extent of malignancy,which indicates that both parameters are involved in the occurrence and development of this malignant tumor.