金耳菌丝体多糖的超声波辅助提取与动力学模型

Ultrasound-assisted Extraction of Polysaccharides from Tremell aurantialba and Its Kinetic Model

为了改善金耳多糖的分离提取技术,利用超声波技术辅助提取金耳菌丝体中的多糖,研究了超声波功率、初始温度、超声波时间和液料比对多糖得率的影响。结果表明:0.5 g菌丝粉于超声波功率600 W、初始温度100℃、超声波时间20 min、提取2次、液料比120∶1(mL∶g)的条件下,多糖的得率最高为11.16%。与传统水浸提法相比,该方法提取效率较高,而且提取时间大大缩短。对最优提取条件下多糖得率随时间的动力学变化分别用Power law模型、Hyperbola模型、Weibull模型和Parabolic diffusion模型进行拟合,通过比较不同模型的决定系数(R2)、偏差因子(BF)和根平均方差(RMSE)发现,所研究的4个模型的拟合度均较好,其中最好的是Weibull模型,其R2值最大且RMSE最小,分别为0.982和0.005 3。

The ultrasound-assisted extraction of polysaccharides from Tremell aurantialba was studied.Effects of different factors,including ultrasonic power,initial temperature,ultrasonic time and the ratio of liquid to material on the polysaccharides yield were studied.The results show that the highest yield of polysaccharides was achieved when 0.5 g T.aurantialba was extracted under conditions of ultrasonic power 600 W,initial temperature 100 ℃,ultrasonic time 20 min,extract 2 times,water-material ratio 120∶1（mL∶g）.Under the above conditions,the yield of polysaccharides was 11.16 %.Comparing with water extraction method,the ultrasound-assisted extraction of polysaccharides from T.aurantialba had advantages of shortening reaction time and improving yield.The yield of polysaccharides at different time were simulated by four kinetic models,i.e.Power law model,Hyperbola model,Weibull model and Parabolic diffusion model.All models gave good simulation to the experimental data based on the analyses of determination coefficient（R2）,bias factor（BF） and root mean square error（RMSE）.The best one was Weibull model with the highest R2 and the smallest RMSE at 0.982 and 0.005 3,respectively.